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Literature summary for 6.1.1.9 extracted from
- Purification and properties of valyl-tRNA synthetase from Mycobacterium smegmatis (1979), Biochim. Biophys. Acta, 568, 253-263.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Penicillamine | - |
Mycolicibacterium smegmatis |  |
| p-hydroxymercuribenzoate | - |
Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
valine | tRNA | Mycolicibacterium smegmatis | |
| 0.00125 | - |
valine | - |
Mycolicibacterium smegmatis | |
| 0.1 | - |
ATP | - |
Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at 5 mM, 16% of the activation with Mg2+ | Mycolicibacterium smegmatis | |
| Mg2+ | optimal concentration: 5 mM | Mycolicibacterium smegmatis | |
| Mn2+ | 60% of the activation with Mg2+ | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000, SDS-PAGE | Mycolicibacterium smegmatis |
| 126000 | - |
gel filtration | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0042 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, stable for several months | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-valine + tRNAVal | tRNA from E. coli or Mycobacterium smegmatis | Mycolicibacterium smegmatis | AMP + diphosphate + L-valyl-tRNAVal | - |
? | |
| additional information | no activity with dATP | Mycolicibacterium smegmatis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 30000, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Mycolicibacterium smegmatis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 min, stable | Mycolicibacterium smegmatis |
| 70 | - |
1 min, complete inactivation | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7 | - |
Mycolicibacterium smegmatis |
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Release 2023.1 (January 2023)
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