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Literature summary for 6.1.1.7 extracted from

  • Chang, C.P.; Chang, C.Y.; Lee, Y.H.; Lin, Y.S.; Wang, C.C.
    Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution (2015), Mol. Cell. Biol., 35, 2242-2253 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ALA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes Tetrapisispora phaffii
gene ALA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes, recombinant expression of GFP-tagged isozyme ALA1 in Saccharomyces cerevisiae strain INVSc1 Vanderwaltozyma polyspora
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes Tetrapisispora phaffii
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes Saccharomyces cerevisiae
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes, recombinant expression of GFP-tagged isozyme ALA2 in Saccharomyces cerevisiae strain INVSc1 Vanderwaltozyma polyspora

Protein Variants

Protein Variants Comment Organism
additional information generation of Saccharomyces cerervisiae ALA1 chimeric fusion proteins with ALA1 and ALA2 from Vanderwaltozyma polyspora and Tetrapisispora phaffii, overview Saccharomyces cerevisiae
additional information generation of Tetrapisispora phaffii ALA1 and ALA2 chimeric fusion proteins with ALA1 from Saccharomyces cerevisiae and Vanderwaltozyma polyspora ALA1 and ALA2, overview Tetrapisispora phaffii
additional information generation of Vanderwaltozyma polyspora ALA1 and ALA2 chimeric fusion proteins with ALA1 from Saccharomyces cerevisiae and Tetrapisispora phaffii, overview Vanderwaltozyma polyspora

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Vanderwaltozyma polyspora 5737
-
cytoplasm
-
Tetrapisispora phaffii 5737
-
cytoplasm
-
Saccharomyces cerevisiae 5737
-
mitochondrion
-
Vanderwaltozyma polyspora 5739
-
mitochondrion
-
Tetrapisispora phaffii 5739
-
additional information the yeast Tetrapisispora phaffii possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart Tetrapisispora phaffii
-
-
additional information the yeast Vanderwaltozyma polyspora possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Clever selection of transcription and translation initiation sites enables the two isoforms to be localized and thus functional in their respective cellular compartments. But the two isoforms can also be stably expressed and function in the reciprocal compartments by insertion or removal of a mitochondrial targeting signal Vanderwaltozyma polyspora
-
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40825
-
-
Tetrapisispora phaffii G8BWM7
-
-
Tetrapisispora phaffii G8C0Z7
-
-
Vanderwaltozyma polyspora A7TPK2 i.e. Kluyveromyces polysporus
-
Vanderwaltozyma polyspora A7TRK4 i.e. Kluyveromyces polysporus
-
Vanderwaltozyma polyspora ATCC 22028 / DSM 70294 A7TPK2 i.e. Kluyveromyces polysporus
-
Vanderwaltozyma polyspora ATCC 22028 / DSM 70294 A7TRK4 i.e. Kluyveromyces polysporus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis Vanderwaltozyma polyspora
recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis Tetrapisispora phaffii
recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
ALA1
-
Vanderwaltozyma polyspora
ALA1
-
Tetrapisispora phaffii
ALA2
-
Vanderwaltozyma polyspora
ALA2
-
Tetrapisispora phaffii
ALA2
-
Saccharomyces cerevisiae
Alanyl-tRNA synthetase
-
Vanderwaltozyma polyspora
Alanyl-tRNA synthetase
-
Tetrapisispora phaffii
Alanyl-tRNA synthetase
-
Saccharomyces cerevisiae
AlaRS
-
Vanderwaltozyma polyspora
AlaRS
-
Tetrapisispora phaffii
AlaRS
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Vanderwaltozyma polyspora
30
-
assay at Tetrapisispora phaffii
30
-
assay at Saccharomyces cerevisiae

Expression

Organism Comment Expression
Vanderwaltozyma polyspora differential regulation of gene expression of ALA genes in yeast species, overview additional information
Tetrapisispora phaffii differential regulation of gene expression of ALA genes in yeast species, overview additional information
Saccharomyces cerevisiae differential regulation of gene expression of ALA genes in yeast species, overview additional information

General Information

General Information Comment Organism
evolution the enzyme belongs to the class IIa aminoacyl-tRNA synthestase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. In contrast, the yeast Tetrapisispora phaffii possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Phylogenetic relationships of yeast AlaRSs, overview Tetrapisispora phaffii
evolution the enzyme belongs to the class IIa aminoacyl-tRNA synthestase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. In contrast, the yeast Vanderwaltozyma polyspora possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Clever selection of transcription and translation initiation sites enables the two isoforms to be localized and thus functional in their respective cellular compartments. But the two isoforms can also be stably expressed and function in the reciprocal compartments by insertion or removal of a mitochondrial targeting signal. Synteny and phylogeny analyses reveal that the AlaRS homologues of Vanderwaltozyma polyspora arose from a dual-functional common ancestor through whole-genome duplication (WGD). Moreover, the mitochondrial form has higher synonymous (1.6fold) and nonsynonymous (2.8fold) substitution rates than does its cytoplasmic counterpart, presumably due to a lesser constraint imposed on components of the mitochondrial translational apparatus. Asymmetric evolution confers the divergence between the AlaRS paralogues of Vanderwaltozyma polyspora. Phylogenetic relationships of yeast AlaRSs, overview Vanderwaltozyma polyspora
evolution the enzyme belongs to the class IIa aminoacyl-tRNA synthestase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. The AlaRS homologues of Saccharomyces cerevisiae arose from a dual-functional common ancestor through whole-genome duplication (WGD), but retains only one copy of the AlaRS gene. Phylogenetic relationships of yeast AlaRSs, overview Saccharomyces cerevisiae
evolution the enzyme belongs to the class IIa aminoacyl-tRNA synthetase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. In contrast, the yeast Tetrapisispora phaffii possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Phylogenetic relationships of yeast AlaRSs, overview Tetrapisispora phaffii