Cloned (Comment) | Organism |
---|---|
gene ALA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes | Tetrapisispora phaffii |
gene ALA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes, recombinant expression of GFP-tagged isozyme ALA1 in Saccharomyces cerevisiae strain INVSc1 | Vanderwaltozyma polyspora |
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes | Tetrapisispora phaffii |
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes | Saccharomyces cerevisiae |
gene ALA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and chimeric fusion enzymes, recombinant expression of GFP-tagged isozyme ALA2 in Saccharomyces cerevisiae strain INVSc1 | Vanderwaltozyma polyspora |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of Saccharomyces cerervisiae ALA1 chimeric fusion proteins with ALA1 and ALA2 from Vanderwaltozyma polyspora and Tetrapisispora phaffii, overview | Saccharomyces cerevisiae |
additional information | generation of Tetrapisispora phaffii ALA1 and ALA2 chimeric fusion proteins with ALA1 from Saccharomyces cerevisiae and Vanderwaltozyma polyspora ALA1 and ALA2, overview | Tetrapisispora phaffii |
additional information | generation of Vanderwaltozyma polyspora ALA1 and ALA2 chimeric fusion proteins with ALA1 from Saccharomyces cerevisiae and Tetrapisispora phaffii, overview | Vanderwaltozyma polyspora |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Vanderwaltozyma polyspora | 5737 | - |
cytoplasm | - |
Tetrapisispora phaffii | 5737 | - |
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
mitochondrion | - |
Vanderwaltozyma polyspora | 5739 | - |
mitochondrion | - |
Tetrapisispora phaffii | 5739 | - |
additional information | the yeast Tetrapisispora phaffii possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart | Tetrapisispora phaffii | - |
- |
additional information | the yeast Vanderwaltozyma polyspora possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Clever selection of transcription and translation initiation sites enables the two isoforms to be localized and thus functional in their respective cellular compartments. But the two isoforms can also be stably expressed and function in the reciprocal compartments by insertion or removal of a mitochondrial targeting signal | Vanderwaltozyma polyspora | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P40825 | - |
- |
Tetrapisispora phaffii | G8BWM7 | - |
- |
Tetrapisispora phaffii | G8C0Z7 | - |
- |
Vanderwaltozyma polyspora | A7TPK2 | i.e. Kluyveromyces polysporus | - |
Vanderwaltozyma polyspora | A7TRK4 | i.e. Kluyveromyces polysporus | - |
Vanderwaltozyma polyspora ATCC 22028 / DSM 70294 | A7TPK2 | i.e. Kluyveromyces polysporus | - |
Vanderwaltozyma polyspora ATCC 22028 / DSM 70294 | A7TRK4 | i.e. Kluyveromyces polysporus | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis | Vanderwaltozyma polyspora |
recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis | Tetrapisispora phaffii |
recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ALA1 | - |
Vanderwaltozyma polyspora |
ALA1 | - |
Tetrapisispora phaffii |
ALA2 | - |
Vanderwaltozyma polyspora |
ALA2 | - |
Tetrapisispora phaffii |
ALA2 | - |
Saccharomyces cerevisiae |
Alanyl-tRNA synthetase | - |
Vanderwaltozyma polyspora |
Alanyl-tRNA synthetase | - |
Tetrapisispora phaffii |
Alanyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
AlaRS | - |
Vanderwaltozyma polyspora |
AlaRS | - |
Tetrapisispora phaffii |
AlaRS | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Vanderwaltozyma polyspora |
30 | - |
assay at | Tetrapisispora phaffii |
30 | - |
assay at | Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Vanderwaltozyma polyspora | differential regulation of gene expression of ALA genes in yeast species, overview | additional information |
Tetrapisispora phaffii | differential regulation of gene expression of ALA genes in yeast species, overview | additional information |
Saccharomyces cerevisiae | differential regulation of gene expression of ALA genes in yeast species, overview | additional information |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the class IIa aminoacyl-tRNA synthestase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. In contrast, the yeast Tetrapisispora phaffii possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Phylogenetic relationships of yeast AlaRSs, overview | Tetrapisispora phaffii |
evolution | the enzyme belongs to the class IIa aminoacyl-tRNA synthestase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. In contrast, the yeast Vanderwaltozyma polyspora possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Clever selection of transcription and translation initiation sites enables the two isoforms to be localized and thus functional in their respective cellular compartments. But the two isoforms can also be stably expressed and function in the reciprocal compartments by insertion or removal of a mitochondrial targeting signal. Synteny and phylogeny analyses reveal that the AlaRS homologues of Vanderwaltozyma polyspora arose from a dual-functional common ancestor through whole-genome duplication (WGD). Moreover, the mitochondrial form has higher synonymous (1.6fold) and nonsynonymous (2.8fold) substitution rates than does its cytoplasmic counterpart, presumably due to a lesser constraint imposed on components of the mitochondrial translational apparatus. Asymmetric evolution confers the divergence between the AlaRS paralogues of Vanderwaltozyma polyspora. Phylogenetic relationships of yeast AlaRSs, overview | Vanderwaltozyma polyspora |
evolution | the enzyme belongs to the class IIa aminoacyl-tRNA synthestase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. The AlaRS homologues of Saccharomyces cerevisiae arose from a dual-functional common ancestor through whole-genome duplication (WGD), but retains only one copy of the AlaRS gene. Phylogenetic relationships of yeast AlaRSs, overview | Saccharomyces cerevisiae |
evolution | the enzyme belongs to the class IIa aminoacyl-tRNA synthetase family. Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. In most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. In contrast, the yeast Tetrapisispora phaffii possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Phylogenetic relationships of yeast AlaRSs, overview | Tetrapisispora phaffii |