Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.7 extracted from

  • Guo, M.; Chong, Y.E.; Shapiro, R.; Beebe, K.; Yang, X.L.; Schimmel, P.
    Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma (2009), Nature, 462, 808-812.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular replacement with the apo-AlaRS structure from Aquifex aeolicus,Protein Data Bank accession code 1RIQ, as the initial model Escherichia coli

Protein Variants

Protein Variants Comment Organism
D235A no improvement in the discrimination between alanine and serine Escherichia coli
D235E no improvement in the discrimination between alanine and serine Escherichia coli
D235N no improvement in the discrimination between alanine and serine Escherichia coli
D235Q no improvement in the discrimination between alanine and serine Escherichia coli
G237A mutation G237A introduces bulk into the alanine-binding pocket, with little other change in the pocket or the surrounding atoms. Shrinking of the alanine-binding pocket sharply raises the Km for alanine but does not greatly perturb the Km for serine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
L-alanine wild-type, 22°C, pH 8.0 Escherichia coli
26
-
glycine wild-type, 22°C, pH 8.0 Escherichia coli
31
-
L-serine wild-type, 22°C, pH 8.0 Escherichia coli
42
-
glycine mutant D235N, 22°C, pH 8.0 Escherichia coli
58
-
L-serine mutant D235N, 22°C, pH 8.0 Escherichia coli
70
-
L-serine mutant G237A, 22°C, pH 8.0 Escherichia coli
73
-
L-alanine mutant G237A, 22°C, pH 8.0 Escherichia coli
88
-
glycine mutant G237A, 22°C, pH 8.0 Escherichia coli
178
-
L-alanine mutant D235N, 22°C, pH 8.0 Escherichia coli
493
-
glycine mutant D235A, 22°C, pH 8.0 Escherichia coli
502
-
L-alanine mutant D235A, 22°C, pH 8.0 Escherichia coli
600
-
L-serine above 600, mutant D235A, 22°C, pH 8.0 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00957
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAAla
-
Escherichia coli AMP + diphosphate + glycyl-tRNAAla
-
?
ATP + L-alanine + tRNAAla
-
Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
?
ATP + L-serine + tRNAAla
-
Escherichia coli AMP + diphosphate + L-seryl-tRNAAla
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0028
-
L-serine mutant G237A, 22°C, pH 8.0 Escherichia coli
0.0035
-
glycine mutant D235A, 22°C, pH 8.0 Escherichia coli
0.0046
-
L-serine mutant D235N, 22°C, pH 8.0 Escherichia coli
0.0058
-
glycine mutant D235N, 22°C, pH 8.0 Escherichia coli
0.0074
-
L-alanine mutant D235N, 22°C, pH 8.0 Escherichia coli
0.069
-
L-alanine mutant D235A, 22°C, pH 8.0 Escherichia coli
1.2
-
L-alanine wild-type, 22°C, pH 8.0 Escherichia coli
1.4
-
L-alanine mutant G237A, 22°C, pH 8.0 Escherichia coli
1.6
-
glycine mutant G237A, 22°C, pH 8.0 Escherichia coli
2.4
-
L-serine wild-type, 22°C, pH 8.0 Escherichia coli
3.7
-
glycine wild-type, 22°C, pH 8.0 Escherichia coli