Cloned (Comment) | Organism |
---|---|
gene ALA1, DNA and amino acid sequence determination and analysis, mitochondrial and cytoplasmic isozymes are encoded by a single nuclear gene ALA1, through alternative use of inframe successive ACG triplets and a downstream AUG triplet, overview, despite participation of the non-AUG-initiated leader peptide in mitochondrial localization, the leader peptide per se cannot target a cytoplasmic passenger protein into mitochondria under normal conditions, functional mapping | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of the cytoplasmic isozyme enables it to overcome the compartmental barrier and function in the mitochondria as well, but deletion of as few as eight amino acid residues from its amino-terminus eliminates such a potential, the sequence upstream of the first in-frame AUG initiator not only carries an unusual initiation site, but also contributes to the pattern of protein expression and localization | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
mitochondrion | very poor mitochondrial targeting activity of the ALA1 leader peptide, mapping of the mitochondrial targeting signal, overview | Saccharomyces cerevisiae | 5739 | - |
additional information | isozyme pattern of expression and localization, overview | Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
mitochondrial and cytoplasmic isozymes encoded by a single nuclear gene ALA1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | isozyme pattern of expression and localization, overview | Saccharomyces cerevisiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alanyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
AlaRS | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |