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Literature summary for 6.1.1.7 extracted from
- kinetic parameters for tmRNA binding to alanyl-tRNA synthetase and elongation factor Tu from Escherichia coli (2000), Biochemistry, 39, 2652-2658.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | elongation factor Tu has no stimulating effect on the enzyme with both substrates: tRNAAla and Ala-tmRNA, but it protects both acylated RNAs against hydrolysis of the alanyl ester bond | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, ternary complex formation | Escherichia coli | |
| 0.0015 | - |
tRNAAla | pH 7.6, 37°C, recombinant His-tagged enzyme | Escherichia coli |  |
| 0.024 | - |
tmRNA | pH 7.6, 37°C, recombinant His-tagged enzyme | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 96000 | - |
1 * 96000, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tRNAAla | Escherichia coli | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
purified recombinant His-tagged enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tmRNA | transfer messenger RNA, 75fold reduced activity compared to cognate tRNAAla | Escherichia coli | AMP + diphosphate + L-alanyl-tmRNA | - |
? | |
| ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
| ATP + L-alanine + tRNAAla | 2-step reaction | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 96000, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ala-tRNA synthetase | - |
Escherichia coli |
| Alanine transfer RNA synthetase | - |
Escherichia coli |
| Alanine translase | - |
Escherichia coli |
| Alanine tRNA synthetase | - |
Escherichia coli |
| Alanine-transfer RNA ligase | - |
Escherichia coli |
| alanine-tRNA ligase | - |
Escherichia coli |
| Alanyl-transfer ribonucleate synthetase | - |
Escherichia coli |
| Alanyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
| Alanyl-transfer RNA synthetase | - |
Escherichia coli |
| Alanyl-tRNA synthetase | - |
Escherichia coli |
| AlaRS | - |
Escherichia coli |
| Synthase, alanyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
tmRNA | pH 7.6, 37°C, 0.001 mg recombinant His-tagged enzyme | Escherichia coli | |
| 0.2 | - |
tRNAAla | pH 7.6, 37°C, 0.00025 mg recombinant His-tagged enzyme | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
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