General Stability | Organism |
---|---|
Zn2+ stabilizes the enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | denaturation at 3.3 M, the Zn2+-depleted enzyme is much more sensitive to denaturation by urea than the native enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | removal of Zn2+ leads to over 50% reduced activity, required for conformational stability and flexibility important for the enzymes activity | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
179000 | - |
Zn2+-depleted enzyme, sedimentation coefficient and analytical ultracentrifugation | Escherichia coli |
186000 | - |
native enzyme, sedimentation coefficient and analytical ultracentrifugation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | Escherichia coli | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
recombinant wild-type from overexpression in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Ala-tRNA synthetase | - |
Escherichia coli |
Alanine transfer RNA synthetase | - |
Escherichia coli |
Alanine translase | - |
Escherichia coli |
Alanine tRNA synthetase | - |
Escherichia coli |
Alanine-transfer RNA ligase | - |
Escherichia coli |
alanine-tRNA ligase | - |
Escherichia coli |
Alanyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Alanyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Alanyl-transfer RNA synthetase | - |
Escherichia coli |
Alanyl-tRNA synthetase | - |
Escherichia coli |
AlaRS | - |
Escherichia coli |
Synthase, alanyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the Zn2+-depleted enzyme shows reduced viscosity at 10°C compared to 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |