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Literature summary for 6.1.1.6 extracted from

  • Ravishankar, S.; Ambady, A.; Swetha, R.G.; Anbarasu, A.; Ramaiah, S.; Sambandamurthy, V.K.
    Essentiality assessment of cysteinyl and lysyl-tRNA synthetases of Mycobacterium smegmatis (2016), PLoS ONE, 11, e0147188 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.6

Application

Application Comment Organism
drug development aminoacyl-tRNA synthetases are attractive targets for the discovery of antibacterial agents Mycolicibacterium smegmatis

Cloned(Commentary)

Cloned (Comment) Organism
gene lysS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155 Mycolicibacterium smegmatis
gene lysS2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information generation of conditional expression strains, conditional expression plasmids are electroporated into Mycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis
additional information generation of conditional expression strains, conditional expression plasmids are electroporated intoMycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane segment analysis of lysyl-tRNA synthetase Mycolicibacterium smegmatis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-lysine + tRNALys Mycolicibacterium smegmatis
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Mycolicibacterium smegmatis mc2155 / ATCC 700084
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0QYV1 gene lysX or lysS2
-
Mycolicibacterium smegmatis A0R577 gene lysS
-
Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0QYV1 gene lysX or lysS2
-
Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0R577 gene lysS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
 Mycolicibacterium smegmatis AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Mycolicibacterium smegmatis mc2155 / ATCC 700084 AMP + diphosphate + L-lysyl-tRNALys
-
 ?

Synonyms

Synonyms Comment Organism
LysRS
-
 Mycolicibacterium smegmatis
lysS
-
 Mycolicibacterium smegmatis
lysS2
-
 Mycolicibacterium smegmatis
LysX
-
 Mycolicibacterium smegmatis
LysX UniProt Mycolicibacterium smegmatis
Lysyl-tRNA synthetase
-
 Mycolicibacterium smegmatis
lysylphosphatidylglycerol biosynthesis bifunctional protein UniProt Mycolicibacterium smegmatis
MSMEG_3796
-
 Mycolicibacterium smegmatis
MSMEG_6094
-
 Mycolicibacterium smegmatis
MSMEI_3707
-
 Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycolicibacterium smegmatis

General Information

General Information Comment Organism
evolution multiple genes encode for aminoacyl-tRNA synthetases from Mycobacterium smegmatis, e.g. glutamyl (GluRS), cysteinyl (CysRS), prolyl (ProRS) and lysyl (LysRS) tRNA synthetases. Conditional expression strains of lysyl-tRNA synthetases generated in Mycobacterium smegmatis reveals that the canonical aminoacyl-tRNA synthetase are essential, while the additional ones are not essential for the growth of Mycobacterium smegmatis. The lysyl-tRNA synthetase of Mycobacterium smegmatis belongs to the class II amino acid tRNA synthetases Mycolicibacterium smegmatis
physiological function canonical LysRS and CysRS are essential for growth of Mycobacterium smegmatis Mycolicibacterium smegmatis
physiological function Essentiality of lysyl and cysteinyl-tRNA synthetases of Mycobacterium smegmatis Mycolicibacterium smegmatis