Activating Compound | Comment | Organism | Structure |
---|---|---|---|
epidermal growth factor receptor | activation of LysRS by epidermal growth factor receptor (EGFR) | Homo sapiens |
Application | Comment | Organism |
---|---|---|
diagnostics | LysRS s207 phosphorylation plays a role as a prognostic factor in lung cancer after primary surgery | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q15046 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the MAPK-ERK pathway leads to the phosphorylation of LysRS on Ser207 and its release from the MSC in activated mast cells. Phosphorylation of Ser207 can be inhibited by U0126 treatment | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
cardiomyocyte | - |
Homo sapiens | - |
HCC-827 cell | - |
Homo sapiens | - |
mast cell | - |
Homo sapiens | - |
additional information | LysRS is ubiquitously found in tissues | Homo sapiens | - |
non-small cell lung carcinoma cell | significantly higher staining levels of phosphos207-LysRS are observed in both the cytoplasm and the nuclei of cancerous tissues compared to healthy tissues | Homo sapiens | - |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is organized in a multi-synthetase complex (MSC) | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
KARS | - |
Homo sapiens |
LysRS | - |
Homo sapiens |
Lysyl-tRNA synthetase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | LysRS enzyme phosphorylation on residue Ser207 is correlated to activity of epidermal growth factor receptor and level of lymph node metastases, e.g. in lung cancer, overview. The MAPK-ERK pathway leads to the phosphorylation of LysRS and its release from the MSC in activated mast cells. Lysyl-tRNA synthetase phosphorylated on Ser207 (P-s207 LysRS) is released from the cytoplasmic multi-tRNA synthetase complex (MSC) into the nucleus, where it activates the microphthalmia-associated transcription factor (MITF) in stimulated cultured mast cells and cardiomyocytes. This specific phosphorylation results in LysRS losing its ability to acetylate tRNA but enhances its production of the second messenger diadenosine tetraphosphate (Ap4A). Ap4A has been shown to bind to the tumor suppressor Hint-1, releasing its inhibition on MITF. Patients with EGFR mutations without lymph node metastases have a higher nuclear expression of Ser207 phosphorylated LysRS (50%) as compared to patients with lymph node metastases (27.8%) | Homo sapiens |
additional information | the enzyme is organized in a multi-synthetase complex (MSC) | Homo sapiens |