Literature summary for 6.1.1.6 extracted from

Nam, S.H.; Kim, D.; Lee, M.S.; Lee, D.; Kwak, T.K.; Kang, M.; Ryu, J.; Kim, H.J.; Song, H.E.; Choi, J.; Lee, G.H.; Kim, S.Y.; Park, S.H.; Kim, D.G.; Kwon, N.H.; Kim, T.Y.; Thiery, J.P.; Kim, S.; Lee, J.W.
Noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels (2015), Oncotarget, 6, 21655-21674 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the complex formation among KRS, p67LR, and integrins alpha6 and beta1 upon cell adhesion can be disrupted by YH16899 treatment. SW620 cells with inhibitors U1026 or YH16899 blocks dissemination. Blockade of dissemination of KRS-suppressed cells is relieved by ERK1/2 and/or paxillin expression	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-
plasma membrane	-	Homo sapiens	5886	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q15046	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
colon cancer cell	-	Homo sapiens	-
HCT-116 cell	-	Homo sapiens	-
SW-620 cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
KRS	-	Homo sapiens
Lysyl-tRNA synthetase	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	the complex formation among KRS, p67LR, and integrins alpha6 and beta1 upon cell adhesion can be disrupted by YH16899 treatment. SW620 cells with inhibitors U1026 or YH16899 blocks dissemination. Blockade of dissemination of KRS-suppressed cells is relieved by ERK1/2 and/or paxillin expression	additional information

General Information

General Information	Comment	Organism
malfunction	KRS-suppressed HCT-116 cells show increased mesenchymal markers and decreased epithelial markers 24 h after embedding of colon cancer spheroids in 3D collagen I gels. KRS-positive cells are sensitive to laminin treatment for p67LR stabilization, KRS-suppressed cells are insensitive to the laminin treatment. KRS-expressing parental cells show disseminative margins that are positive for E-cadherin expression, whereas KRS-suppressed cells show neither. Specific effect of KRS suppression on paxillin. KRS suppression decreases the phosphorylations of vinculin Tyr822 and tensin2 Tyr483, but not of talin Ser425. KRS-suppression decreases ERK1/2 phosphorylation, ERK1/2 phosphorylation is increased by KRS overexpression. Treating SW620 cells with inhibitors U1026 or YH16899 blocks dissemination	Homo sapiens
additional information	noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels	Homo sapiens
physiological function	KRS at the plasma membrane is important for cancer metastasis, canonical roles of cytosolic KRS in protein translation. KRS and its downstream effectors promote the metastatic migration. Complex formation among KRS, p67LR, and integrins alpha6 and beta1 upon cell adhesion, KRS/p67LR/integrin alpha6beta1 linkage correlates for ERK1/2 activation, KRS-dependent ERK1/2 activation. KRS has prometastatic roles at the invasive margins of KRS-/+ mouse breast tumor and human colon tumor tissues	Homo sapiens

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Release 2023.1 (January 2023)