Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
ATP + L-lysine + tRNALys | Saccharomyces cerevisiae YPH499 | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32048 | - |
- |
Saccharomyces cerevisiae YPH499 | P32048 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
ATP + L-lysine + tRNALys | - |
Saccharomyces cerevisiae YPH499 | AMP + diphosphate + L-lysyl-tRNALys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FOSTERSO_4045 | - |
Saccharomyces cerevisiae |
Lysyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
mitochondrial lysyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Msk1p | - |
Saccharomyces cerevisiae |
preMsk1p | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | in yeast, the import of tRNALys with CUU anticodon (tRK1) relies on a complex mechanism where interaction with enolase 2 (Eno2p) dictates a deep conformational change of the tRNA. This event is believed to mask the tRNA from the cytosolic translational machinery to redirect it towards the mitochondria. Once near the mitochondrial outer membrane, the precursor of the mitochondrial lysyl-tRNA synthetase (preMsk1p) takes over enolase to carry the tRNA within the mitochondrial matrix, where it is supposed to participate in translation following correct refolding. Mechanism of mitochondrial import of the yeast lysine isoacceptor tRNA with a CUU anticodon (tRK1). tRK1 in the canonical Lform undertakes refolding (Fform) through interaction mainly with enolase 2 (Eno2p), overview. While about 95% of the tRK1 cellular pool is used in cytosolic translation, this complex shuttles the remaining about 5% to the mitochondrial surface, where it interacts with preMsk1p, which proceeds to import properly. The mitochondrial targeting sequence (MTS) is displayed as an additional appendage bound to the N-terminal end of the protein representation | Saccharomyces cerevisiae |