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Literature summary for 6.1.1.6 extracted from

  • Ofir-Birin, Y.; Fang, P.; Bennett, S.P.; Zhang, H.M.; Wang, J.; Rachmin, I.; Shapiro, R.; Song, J.; Dagan, A.; Pozo, J.; Kim, S.; Marshall, A.G.; Schimmel, P.; Yang, X.L.; Nechushtan, H.; Razin, E.; Guo, M.
    Structural switch of lysyl-tRNA synthetase between translation and transcription (2013), Mol. Cell, 49, 30-42.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with p38/AIMP2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
S270D the mutant shows 800fold enhanced catalytic activity compared to the wild type enzyme, with enhanced diadenosine tetraphosphate synthetic activity, enhanced ATP hydrolysis and lost aminoacylation activity for tRNALys Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-lysine + tRNALys Homo sapiens
-
AMP + diphosphate + L-lysyl-tRNALys
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Ser207-phosphorylation inactivates the enzyme translationally, but activates its transcriptional function Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
Homo sapiens AMP + diphosphate + L-lysyl-tRNALys
-
?

Synonyms

Synonyms Comment Organism
LysRS
-
Homo sapiens
Lysyl-tRNA synthetase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens