Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
CAT W314F | mutant of the truncated C-terminal catalytic domain CAT | Geobacillus stearothermophilus |
CAT W332F | mutant of the truncated C-terminal catalytic domain CAT | Geobacillus stearothermophilus |
GsLysRS W314F | mutant of Geobacillus stearothermophilus lysyl-tRNA synthetase | Geobacillus stearothermophilus |
GsLysRS W332F | mutant of Geobacillus stearothermophilus lysyl-tRNA synthetase | Geobacillus stearothermophilus |
truncated N-terminal tRNA anticodon-binding domain | TAB | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
wild-type CAT and CAT mutants, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Geobacillus stearothermophilus | - |
AMP + diphosphate + L-lysyl-tRNALys | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9RHV9 | - |
- |
Purification (Comment) | Organism |
---|---|
CAT mutants are purified to homogeneity | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-lysyl-tRNALys | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | CAT mutants and wild-type CAT | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
GsLysRS | - |
Geobacillus stearothermophilus |
Lysyl-tRNA synthetase | - |
Geobacillus stearothermophilus |