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Literature summary for 6.1.1.6 extracted from

  • Mahapatra, A.; Srinivasan, G.; Richter, K.B.; Meyer, A.; Lienard, T.; Zhang, J.K.; Zhao, G.; Kang, P.T.; Chan, M.; Gottschalk, G.; Metcalf, W.W.; Krzycki, J.A.
    Class I and class II lysyl-tRNA synthetase mutants and the genetic encoding of pyrrolysine in Methanosarcina spp. (2007), Mol. Microbiol., 64, 1306-1318.
    View publication on PubMed
Search for more literature for 6.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanosarcina acetivorans
expressed in Escherichia coli BL21(DE3) cells Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans
-
-
-
Methanosarcina barkeri Q9C4B9
-
-
Methanosarcina barkeri MS / DSM 800 Q9C4B9
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography Methanosarcina acetivorans
nickel-affinity column chromatography Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALysCUU
-
 Methanosarcina acetivorans AMP + L-lysyl-tRNALysCUU + diphosphate
-
 ?
ATP + L-lysine + tRNALysCUU
-
 Methanosarcina barkeri AMP + L-lysyl-tRNALysCUU + diphosphate
-
 ?
ATP + L-lysine + tRNALysCUU
-
 Methanosarcina barkeri MS / DSM 800 AMP + L-lysyl-tRNALysCUU + diphosphate
-
 ?
ATP + L-lysine + tRNALysUUU
-
 Methanosarcina acetivorans AMP + L-lysyl-tRNALysUUU + diphosphate
-
 ?
ATP + L-lysine + tRNALysUUU
-
 Methanosarcina barkeri AMP + L-lysyl-tRNALysUUU + diphosphate
-
 ?
ATP + L-lysine + tRNALysUUU
-
 Methanosarcina barkeri MS / DSM 800 AMP + L-lysyl-tRNALysUUU + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
class I lysyl-tRNA synthetase
-
 Methanosarcina acetivorans
class I lysyl-tRNA synthetase
-
 Methanosarcina barkeri
class II lysyl-tRNA synthetase
-
 Methanosarcina acetivorans
class II lysyl-tRNA synthetase
-
 Methanosarcina barkeri
LysRS1
-
 Methanosarcina acetivorans
LysRS1
-
 Methanosarcina barkeri
LysRS2
-
 Methanosarcina acetivorans
LysRS2
-
 Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanosarcina acetivorans
ATP
-
 Methanosarcina barkeri