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Literature summary for 6.1.1.6 extracted from

  • Wang, S.; Praetorius-Ibba, M.; Ataide, S.F.; Roy, H.; Ibba, M.
    Discrimination of cognate and noncognate substrates at the active site of class I lysyl-tRNA synthetase (2006), Biochemistry, 45, 3646-3652.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Borreliella burgdorferi

Protein Variants

Protein Variants Comment Organism
E43I significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
E43Q significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
G29A significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
H242A significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
H242L shows almost no change in the catalytic activity compared to the wild type enzyme Borreliella burgdorferi
H242W significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
T31G significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
T31S shows almost no change in the catalytic activity compared to the wild type enzyme Borreliella burgdorferi
W220A significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
W220L significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
W220Y significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi
Y269F 6fold drop in the catalytic efficiency compared to the wild type enzyme Borreliella burgdorferi
Y269S significant decrease of catalytic activity compared to the wild type enzyme Borreliella burgdorferi

Inhibitors

Inhibitors Comment Organism Structure
S-(2-aminoethyl)-L-cysteine competitive inhibitor Borreliella burgdorferi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 L-lysine mutant enzyme H242L Borreliella burgdorferi
0.23
-
 L-lysine wild type enzyme Borreliella burgdorferi
0.33
-
 L-lysine mutant enzyme W220A Borreliella burgdorferi
0.4
-
 L-lysine mutant enzyme Y269F Borreliella burgdorferi
0.76
-
 L-lysine mutant enzyme T31S Borreliella burgdorferi
0.83
-
 tRNALysUUU mutant enzyme Y269F Borreliella burgdorferi
1
-
 tRNALysUUU mutant enzyme H242A Borreliella burgdorferi
1.1
-
 tRNALysUUU mutant enzyme W220L Borreliella burgdorferi
1.1
-
 tRNALysUUU mutant enzyme Y269S Borreliella burgdorferi
1.2
-
 tRNALysUUU mutant enzyme W220Y Borreliella burgdorferi
1.3
-
 ATP wild type enzyme Borreliella burgdorferi
1.3
-
 tRNALysUUU mutant enzyme H242L Borreliella burgdorferi
1.6
-
 ATP mutant enzyme Y269F Borreliella burgdorferi
1.7
-
 ATP mutant enzyme T31S Borreliella burgdorferi
2
-
 tRNALysUUU wild type enzyme Borreliella burgdorferi
2.1
-
 tRNALysUUU mutant enzyme T31G Borreliella burgdorferi
2.1
-
 tRNALysUUU mutant enzyme T31S Borreliella burgdorferi
2.5
-
 tRNALysUUU mutant enzyme W220A Borreliella burgdorferi
2.8
-
 L-lysine mutant enzyme Y269S Borreliella burgdorferi
3.3
-
 L-lysine mutant enzyme H242A Borreliella burgdorferi
3.3
-
 L-lysine mutant enzyme W220L Borreliella burgdorferi
4.4
-
 L-lysine mutant enzyme T31G Borreliella burgdorferi
4.7
-
 L-lysine mutant enzyme W220Y Borreliella burgdorferi
5.1
-
 tRNALysUUU mutant enzyme G29A Borreliella burgdorferi
6.3
-
 L-lysine mutant enzyme G29A Borreliella burgdorferi
6.9
-
 ATP mutant enzyme Y269S Borreliella burgdorferi
9.5
-
 ATP mutant enzyme T31G Borreliella burgdorferi

Organism

Organism UniProt Comment Textmining
Borreliella burgdorferi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Superose 6 column chromatography Borreliella burgdorferi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALysUUU
-
 Borreliella burgdorferi AMP + L-lysyl-tRNALysUUU + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
class I lysyl-tRNA synthetase
-
 Borreliella burgdorferi
LysRS1
-
 Borreliella burgdorferi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00012
-
 L-lysine mutant enzyme H242A Borreliella burgdorferi
0.00012
-
 tRNALysUUU mutant enzyme W220L Borreliella burgdorferi
0.00013
-
 tRNALysUUU mutant enzyme H242A Borreliella burgdorferi
0.00017
-
 L-lysine mutant enzyme W220L Borreliella burgdorferi
0.00051
-
 ATP mutant enzyme Y269S Borreliella burgdorferi
0.00056
-
 tRNALysUUU mutant enzyme W220A Borreliella burgdorferi
0.00075
-
 L-lysine mutant enzyme W220A Borreliella burgdorferi
0.0011
-
 ATP mutant enzyme T31G Borreliella burgdorferi
0.0023
-
 tRNALysUUU mutant enzyme Y269S Borreliella burgdorferi
0.0026
-
 L-lysine mutant enzyme Y269S Borreliella burgdorferi
0.0046
-
 L-lysine mutant enzyme T31G Borreliella burgdorferi
0.005
-
 tRNALysUUU mutant enzyme T31G Borreliella burgdorferi
0.017
-
 ATP mutant enzyme Y269F Borreliella burgdorferi
0.099
-
 L-lysine mutant enzyme Y269F Borreliella burgdorferi
0.13
-
 L-lysine mutant enzyme W220Y Borreliella burgdorferi
0.14
-
 tRNALysUUU mutant enzyme Y269F Borreliella burgdorferi
0.23
-
 tRNALysUUU mutant enzyme W220Y Borreliella burgdorferi
0.29
-
 L-lysine mutant enzyme H242L Borreliella burgdorferi
0.29
-
 tRNALysUUU mutant enzyme H242L Borreliella burgdorferi
0.33
-
 tRNALysUUU wild type enzyme Borreliella burgdorferi
0.34
-
 L-lysine wild type enzyme Borreliella burgdorferi
0.45
-
 L-lysine mutant enzyme G29A Borreliella burgdorferi
0.53
-
 L-lysine mutant enzyme T31S Borreliella burgdorferi
0.57
-
 ATP wild type enzyme Borreliella burgdorferi
0.59
-
 tRNALysUUU mutant enzyme G29A Borreliella burgdorferi
0.68
-
 tRNALysUUU mutant enzyme T31S Borreliella burgdorferi
1.7
-
 ATP mutant enzyme T31S Borreliella burgdorferi

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Borreliella burgdorferi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.9
-
 S-(2-aminoethyl)-L-cysteine mutant enzyme H242L Borreliella burgdorferi
3.6
-
 S-(2-aminoethyl)-L-cysteine wild type enzyme Borreliella burgdorferi
8.5
-
 S-(2-aminoethyl)-L-cysteine mutant enzyme H242A Borreliella burgdorferi
11
-
 S-(2-aminoethyl)-L-cysteine mutant enzyme W220L Borreliella burgdorferi
11.8
-
 S-(2-aminoethyl)-L-cysteine mutant enzyme G29A Borreliella burgdorferi
17
-
 S-(2-aminoethyl)-L-cysteine mutant enzyme W220Y Borreliella burgdorferi
19
-
 S-(2-aminoethyl)-L-cysteine mutant enzyme W220A Borreliella burgdorferi