Cloned (Comment) | Organism |
---|---|
COS7 cells are cotransfected with a plasmid containing HIV-1 proviral DNA and a plasmid containing cDNA coding for either full-length human LysRS or a truncated LysRS variant in which the N-terminal 65 amino acids have been deleted (DELTA1-65 LysRS). N-terminally truncated LysRS, which binds poorly to tRNALys, does not increase tRNALys packaging into viruses, while C-terminally truncated LysRS (DELTA452-597), which binds to but does not aminoacylate tRNALys, still facilitates an increase in tRNALys packaging into virions | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-65 | mutant enzyme binds poorly to tRNALys, but does not increase tRNALys packaging into HIV-1 viruses | Homo sapiens |
DELTA452-597 | mutant enzyme binds to but does not aminoacylate tRNALys, still facilitates an increase in tRNALys packaging into virions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | - |
Homo sapiens | AMP + L-lysyl-tRNALys + diphosphate | - |
? |