Cloned (Comment) | Organism |
---|---|
DNA sequence determination | Aeropyrum pernix |
expression in strain BL21(DE3) as His-tagged protein | Escherichia coli |
gene lysS, constitutive expression in Escherichia coli DH5alpha, functional complementation of a class II-enzyme-deficient Escherichia coli mutant only with addition of exogenous lysine | Methanococcus maripaludis |
gene lysS, constitutive expression in Escherichia coli DH5alpha, functional complementation of a class II-enzyme-deficient Escherichia coli mutant up to 40°C growth temperature with or without addition of exogenous lysine | Borreliella burgdorferi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CNBr | treatment of tRNALys substrate with CNBr reduces the enzyme activity | Methanococcus maripaludis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
Escherichia coli wild type tRNA | recombinant enzyme, pH 7.2, 37°C | Borreliella burgdorferi | |
0.0019 | - |
Escherichia coli wild type tRNA | recombinant enzyme, pH 7.2, 37°C | Escherichia coli | |
0.002 | - |
Borellia burgdorferi tRNALys | recombinant enzyme, pH 7.2, 37°C | Borreliella burgdorferi | |
0.0039 | - |
Escherichia coli G2.U71 tRNA | recombinant enzyme, pH 7.2, 37°C | Borreliella burgdorferi | |
0.0043 | - |
Borellia burgdorferi tRNALys | recombinant enzyme, pH 7.2, 37°C | Escherichia coli | |
0.0063 | - |
Escherichia coli G2.U71 tRNA | recombinant enzyme, pH 7.2, 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Escherichia coli | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | Methanococcus maripaludis | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | Borreliella burgdorferi | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | Aeropyrum pernix | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YFT9 | class I enzyme | - |
Borreliella burgdorferi | - |
class I enzyme, purified recombinant enzyme expressed in Escherichia coli | - |
Escherichia coli | - |
class II enzyme | - |
Methanococcus maripaludis | - |
class I enzyme, purified recombinant enzyme expressed in Escherichia coli DH5alpha | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from stran BL21(DE3), functional complementation of a class II-enzyme-deficient Escherichia coli mutant | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + Borellia burgdorferi tRNALys | - |
Escherichia coli | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
ATP + L-lysine + Borellia burgdorferi tRNALys | - |
Borreliella burgdorferi | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
ATP + L-lysine + Escherichia coli G2.U71 tRNA | - |
Escherichia coli | ? | - |
? | |
ATP + L-lysine + Escherichia coli G2.U71 tRNA | - |
Borreliella burgdorferi | ? | - |
? | |
ATP + L-lysine + Escherichia coli G2.U71 tRNALys | - |
Escherichia coli | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
ATP + L-lysine + Escherichia coli G2.U71 tRNALys | - |
Borreliella burgdorferi | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
ATP + L-lysine + Escherichia coli tRNALys | - |
Methanococcus maripaludis | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
ATP + L-lysine + Escherichia coli tRNALys CNBr-treated | - |
Methanococcus maripaludis | AMP + diphosphate + L-lysyl-tRNALys CNBr-treated | - |
r | |
ATP + L-lysine + Escherichia coli wild type tRNA | - |
Escherichia coli | ? | - |
? | |
ATP + L-lysine + Escherichia coli wild type tRNA | - |
Borreliella burgdorferi | ? | - |
? | |
ATP + L-lysine + Escherichia coli wild type tRNALys | - |
Escherichia coli | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
ATP + L-lysine + Escherichia coli wild type tRNALys | - |
Borreliella burgdorferi | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
ATP + L-lysine + Methanococcus maripaludis tRNALys | - |
Methanococcus maripaludis | AMP + diphosphate + L-lysyl-tRNALys | - |
r | |
ATP + L-lysine + Methanococcus maripaludis tRNALys CNBr-treated | - |
Methanococcus maripaludis | AMP + diphosphate + L-lysyl-tRNALys CNBr-treated | - |
r | |
ATP + L-lysine + tRNALys | - |
Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | - |
Methanococcus maripaludis | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | - |
Borreliella burgdorferi | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | - |
Aeropyrum pernix | AMP + L-lysyl-tRNALys + diphosphate | - |
r | |
ATP + L-lysine + tRNALys | predicted secondary structure of the tRNALys from Methanococcus maripaludis | Methanococcus maripaludis | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | substrate recognition mechanism | Aeropyrum pernix | AMP + L-lysyl-tRNALys + diphosphate | - |
r | |
ATP + L-lysine + tRNALys | tRNA substrate from Borrelia burgdorferi or Escherichia coli, predicted secondary structure of the tRNALys from Borrelia burgdorferi | Borreliella burgdorferi | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | tRNA substrate from Borrelia burgdorferi or Escherichia coli, predicted secondary structure of tRNAlys from Escherichia coli | Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
additional information | substrate recognition specificity, the enzyme also performs the ATP-diphosphate exchange reaction, class I lysine-tRNA synthetases recognize the same elements in tRNALys as their class II counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes | Borreliella burgdorferi | ? | - |
? | |
additional information | substrate recognition specificity, the enzyme also performs the ATP-diphosphate exchange reaction, class II lysine-tRNA synthetases recognize the same elements in tRNALys as their class I counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes | Escherichia coli | ? | - |
? | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction, class I lysine-tRNA synthetases recognize the same elements in tRNALys as their class II counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes | Methanococcus maripaludis | ? | - |
? | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction, class I lysine-tRNA synthetases recognize the same elements in tRNALys as their class II counterparts, namely the discriminator base N73 and the anticodon, but vary in the recognition of the G2.U71 wobble pair of spirochete tRNALys, which acts as a determinant for class II enzymes, but not for class I enzymes | Aeropyrum pernix | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Escherichia coli |
L-Lysine-transfer RNA ligase | - |
Methanococcus maripaludis |
L-Lysine-transfer RNA ligase | - |
Borreliella burgdorferi |
L-Lysine-transfer RNA ligase | - |
Aeropyrum pernix |
Lysine translase | - |
Escherichia coli |
Lysine translase | - |
Methanococcus maripaludis |
Lysine translase | - |
Borreliella burgdorferi |
Lysine translase | - |
Aeropyrum pernix |
Lysine--tRNA ligase | - |
Escherichia coli |
Lysine--tRNA ligase | - |
Methanococcus maripaludis |
Lysine--tRNA ligase | - |
Borreliella burgdorferi |
Lysine--tRNA ligase | - |
Aeropyrum pernix |
Lysine-tRNA synthetase | - |
Escherichia coli |
Lysine-tRNA synthetase | - |
Methanococcus maripaludis |
Lysine-tRNA synthetase | - |
Borreliella burgdorferi |
Lysine-tRNA synthetase | - |
Aeropyrum pernix |
LysRS | - |
Escherichia coli |
LysRS | - |
Methanococcus maripaludis |
LysRS | - |
Borreliella burgdorferi |
LysRS | - |
Aeropyrum pernix |
Lysyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Lysyl-transfer ribonucleate synthetase | - |
Methanococcus maripaludis |
Lysyl-transfer ribonucleate synthetase | - |
Borreliella burgdorferi |
Lysyl-transfer ribonucleate synthetase | - |
Aeropyrum pernix |
Lysyl-transfer RNA synthetase | - |
Escherichia coli |
Lysyl-transfer RNA synthetase | - |
Methanococcus maripaludis |
Lysyl-transfer RNA synthetase | - |
Borreliella burgdorferi |
Lysyl-transfer RNA synthetase | - |
Aeropyrum pernix |
Lysyl-tRNA synthetase | - |
Escherichia coli |
Lysyl-tRNA synthetase | - |
Methanococcus maripaludis |
Lysyl-tRNA synthetase | - |
Borreliella burgdorferi |
Lysyl-tRNA synthetase | - |
Aeropyrum pernix |
Synthetase, lysyl-transfer ribonucleate | - |
Escherichia coli |
Synthetase, lysyl-transfer ribonucleate | - |
Methanococcus maripaludis |
Synthetase, lysyl-transfer ribonucleate | - |
Borreliella burgdorferi |
Synthetase, lysyl-transfer ribonucleate | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Methanococcus maripaludis |
37 | - |
assay at | Borreliella burgdorferi |
37 | - |
assay at | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Escherichia coli tRNALys CNBr-treated | recombinant enzyme, pH 7.2, 37°C | Methanococcus maripaludis | |
0.0025 | - |
Escherichia coli G2.U71 tRNA | recombinant enzyme, pH 7.2, 37°C | Escherichia coli | |
0.0035 | - |
Borellia burgdorferi tRNALys | recombinant enzyme, pH 7.2, 37°C | Escherichia coli | |
0.0035 | - |
Escherichia coli G2.U71 tRNA | recombinant enzyme, pH 7.2, 37°C | Borreliella burgdorferi | |
0.004 | - |
Escherichia coli tRNALys | recombinant enzyme, pH 7.2, 37°C | Methanococcus maripaludis | |
0.004 | - |
Escherichia coli wild type tRNALys | recombinant enzyme, pH 7.2, 37°C | Borreliella burgdorferi | |
0.05 | - |
Methanococcus maripaludis tRNALys CNBr-treated | recombinant enzyme, pH 7.2, 37°C | Methanococcus maripaludis | |
0.055 | - |
Escherichia coli wild type tRNALys | recombinant enzyme, pH 7.2, 37°C | Escherichia coli | |
0.2 | - |
Methanococcus maripaludis tRNALys | recombinant enzyme, pH 7.2, 37°C | Methanococcus maripaludis | |
0.79 | - |
Borellia burgdorferi tRNALys | recombinant enzyme, pH 7.2, 37°C | Borreliella burgdorferi | |
6.08 | - |
Borellia burgdorferi tRNALys | recombinant enzyme, pH 7.2, 37°C | Borreliella burgdorferi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli |
7.2 | - |
assay at | Methanococcus maripaludis |
7.2 | - |
assay at | Borreliella burgdorferi |
7.2 | - |
assay at | Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Methanococcus maripaludis | |
ATP | - |
Borreliella burgdorferi | |
ATP | - |
Aeropyrum pernix |