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Literature summary for 6.1.1.5 extracted from

  • Yang, J.A.; Park, D.W.; Sohn, J.W.; Yang, I.S.; Kim, K.H.; Kim, M.J.
    Molecular analysis of isoleucyl-tRNA synthetase mutations in clinical isolates of methicillin-resistant Staphylococcus aureus with low-level mupirocin resistance (2006), J. Korean Med. Sci., 21, 827-832.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
analysis of the crystal structure with bound muciproin, PDB ID: 1FFY Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
F227L the naturally occuring mutation affects the muciprocin binding Staphylococcus aureus
K226T the naturally occuring mutation affects the muciprocin binding Staphylococcus aureus
P187F the naturally occuring mutation affects the muciprocin binding Staphylococcus aureus
Q612H the naturally occuring mutation is involved in stabilizing the conformation of the catalytic loop containing the KMSKS motif Staphylococcus aureus
V588F the naturally occuring mutation affects the Rossman fold and leads to low-level mupirocin resistance Staphylococcus aureus
V767D the naturally occuring mutation affects the muciprocin binding Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
mupirocin a specific inhibitor of IleRS, which binds in the vicinity of an ATP-binding subsite, and is a bifunctional inhibitor with characteristics of both isoleucine and ATP, i.e. an analogue of isoleucyladenylate, binding structure, overview, mupirocin resistance is phenotypically divided into two groups: low-level and high-level. Highlevel resistance is mediated by a plasmid containing the ileS-2 gene that encodes a distinct isoleucyl-tRNA synthetase enzyme, whereas low-level resistance usually results from alteration of the native IleS as a consequence of spontaneous mutations in the ileS gene Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-isoleucine + tRNAIle Staphylococcus aureus
-
AMP + diphosphate + L-isoleucyl-tRNAIle
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus P41972 methicillin-resistant clinical isolates from humans, gene ileS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-isoleucine + tRNAIle
-
Staphylococcus aureus AMP + diphosphate + L-isoleucyl-tRNAIle
-
?

Synonyms

Synonyms Comment Organism
IleRS
-
Staphylococcus aureus
Isoleucyl-tRNA synthetase
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Staphylococcus aureus