Crystallization (Comment) | Organism |
---|---|
analysis of the crystal structure with bound muciproin, PDB ID: 1FFY | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
F227L | the naturally occuring mutation affects the muciprocin binding | Staphylococcus aureus |
K226T | the naturally occuring mutation affects the muciprocin binding | Staphylococcus aureus |
P187F | the naturally occuring mutation affects the muciprocin binding | Staphylococcus aureus |
Q612H | the naturally occuring mutation is involved in stabilizing the conformation of the catalytic loop containing the KMSKS motif | Staphylococcus aureus |
V588F | the naturally occuring mutation affects the Rossman fold and leads to low-level mupirocin resistance | Staphylococcus aureus |
V767D | the naturally occuring mutation affects the muciprocin binding | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mupirocin | a specific inhibitor of IleRS, which binds in the vicinity of an ATP-binding subsite, and is a bifunctional inhibitor with characteristics of both isoleucine and ATP, i.e. an analogue of isoleucyladenylate, binding structure, overview, mupirocin resistance is phenotypically divided into two groups: low-level and high-level. Highlevel resistance is mediated by a plasmid containing the ileS-2 gene that encodes a distinct isoleucyl-tRNA synthetase enzyme, whereas low-level resistance usually results from alteration of the native IleS as a consequence of spontaneous mutations in the ileS gene | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | Staphylococcus aureus | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | P41972 | methicillin-resistant clinical isolates from humans, gene ileS | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Staphylococcus aureus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IleRS | - |
Staphylococcus aureus |
Isoleucyl-tRNA synthetase | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |