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Literature summary for 6.1.1.4 extracted from
- Crystallization and preliminary X-ray crystallographic study of leucyl-tRNA synthetase from the archaeon Pyrococcus horikoshii (2004), Acta Crystallogr. Sect. D, 60, 1916-1918.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in a C-terminally truncated form in Escherichia coli | Pyrococcus horikoshii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = b = 186.20, c = 91.43 A, alpha = beta = 90, gamma = 120°. The asymmetric unit contains one molecule of LeuRS, with a corresponding crystal volume per protein weight of 3.2 A3 Da(-1) and a solvent content of 60.7%. A data set diffracting to 2.2 A resolution is collected from a single crystal at -173°C. Selenomethionine-substituted protein crystals are prepared in order to solve the structure by the SAD phasing method | Pyrococcus horikoshii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 113000 | - |
x * 113000, calculated from sequence | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus horikoshii |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 113000, calculated from sequence | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Leucyl-tRNA synthetase | - |
Pyrococcus horikoshii |
| LeuRS | - |
Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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