Protein Variants | Comment | Organism |
---|---|---|
R449A | site-directed mutagenesis, nearly inactive mutant | Saccharomyces cerevisiae |
R449E | site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 | Saccharomyces cerevisiae |
R449K | site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320, 30fold reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
R451A | site-directed mutagenesis, nearly inactive mutant | Saccharomyces cerevisiae |
R451E | site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 | Saccharomyces cerevisiae |
R451K | site-directed mutagenesis, mutation within the RDW peptide, complementation of the null mutant strain QBY320, 11fold reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
W445A | site-directed mutagenesis, nearly inactive mutant | Saccharomyces cerevisiae |
W445F | site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 | Saccharomyces cerevisiae |
W445H | site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 | Saccharomyces cerevisiae |
W445K | site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 | Saccharomyces cerevisiae |
W445Y | site-directed mutagenesis, mutation within the RDW peptide, weak complementation of the null mutant strain QBY320, 30fold reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes | Saccharomyces cerevisiae | |
0.0002 | - |
tRNALeu | mutant R449K | Saccharomyces cerevisiae | |
0.0005 | - |
tRNALeu | mutant W445Y | Saccharomyces cerevisiae | |
0.0006 | - |
tRNALeu | wild-type enzyme | Saccharomyces cerevisiae | |
0.0007 | - |
tRNALeu | mutant R451K | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | a two step reaction, the first of which is reversible, aminoacylation and editing by LeuRS require migration of the tRNA acceptor stem end between the canonical aminoacylation core and a separate domain called CP1 that is responsible for amino acid editing, post-transfer editing mechanism., overview | Saccharomyces cerevisiae | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
additional information | the LeuRS CP1 domain can also support group I intron RNA splicing in the yeast mitochondria, overview, the RDW peptide, a highly conserved peptide within an RDW-containing motif, is important for enzyme interactions, the RDW peptide is dynamic and forms unique sets of interactions with the aminoacylation and editing complexes, overview | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | primary and tertiary structure, overview, the RDW peptide, a highly conserved peptide within an RDW-containing motif, is important for enzyme interactions, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Leucyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
LeuRS | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
tRNALeu | mutant R449K | Saccharomyces cerevisiae | |
0.2 | - |
tRNALeu | mutant W445Y | Saccharomyces cerevisiae | |
0.6 | - |
tRNALeu | mutant R451K | Saccharomyces cerevisiae | |
5 | - |
tRNALeu | wild-type enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |