Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Escherichia coli | |
DTT | - |
Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Escherichia coli |
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
K809A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
K846A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-typ enzyme | Escherichia coli |
K846A/K853A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
K846E | site-directed mutagenesis, the mutant shows similar activity compared to the wild-typ enzyme | Escherichia coli |
K846E/K853E | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
K853A | site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-typ enzyme | Escherichia coli |
K853E | site-directed mutagenesis, the mutant shows increased activity compared to the wild-typ enzyme | Escherichia coli |
L854A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-typ enzyme | Escherichia coli |
L855A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
additional information | deletions of the C terminus differentially impact the two functions of the enzyme in splicing and aminoacylation in vivo, overview, a five-amino acid C-terminal deletion of LeuRS, which does not complement a null strain, can form a ternary complex with the bI4 intron and its maturase splicing partner, however, the complex fails to stimulate splicing activity, deletion of the C-terminal domain of LeuRS abolishes aminoacylation of tRNALeu and also amino acid editing of mischarged tRNA molecules, overview | Escherichia coli |
additional information | deletions of the C terminus differentially impact the two functions of the enzyme in splicing and aminoacylation in vivo, overview, a five-amino acid C-terminal deletion of LeuRS, which does not complement a null strain, can form a ternary complex with the bI4 intron and its maturase splicing partner, however, the complex fails to stimulate splicing activity, deletion of the entire yeast mitochondrial LeuRS C-terminal domain enhances its aminoacylation and amino acid editing activities | Saccharomyces cerevisiae |
N807A | site-directed mutagenesis, the mutant shows similar activity compared to the wild-typ enzyme | Escherichia coli |
N807A/N856A | site-directed mutagenesis, the mutant shows similar activity compared to the wild-typ enzyme | Escherichia coli |
N856A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-typ enzyme | Escherichia coli |
Q805A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
Q805A/N807A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
Q805A/N807A/N856A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
R811A | site-directed mutagenesis, the mutant shows decreased activity compared to the wild-typ enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Escherichia coli | two functions of the enzyme in splicing and aminoacylation in vivo, overview | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | Saccharomyces cerevisiae | two functions of the enzyme in splicing and aminoacylation in vivo, overview | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | two functions of the enzyme in splicing and aminoacylation in vivo, overview | Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | two functions of the enzyme in splicing and aminoacylation in vivo, overview | Saccharomyces cerevisiae | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | primary and tertiary structures of the LeuRS unique C-terminal domain, overview, the C-terminal extension of about 60 amino acids forms a discrete domain, which is unique among the LeuRSs and interacts with the corner of the L-shaped tRNALeu, overview | Escherichia coli |
More | primary and tertiary structures of the LeuRS unique C-terminal domain, overview, the C-terminal extension of about 60 amino acids forms a discrete domain, which is unique among the LeuRSs and interacts with the corner of the L-shaped tRNALeu, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Leucyl-tRNA synthetase | - |
Escherichia coli |
Leucyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
LeuRS | - |
Escherichia coli |
LeuRS | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Saccharomyces cerevisiae |