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Literature summary for 6.1.1.4 extracted from

  • Lue, S.W.; Kelley, S.O.
    A single residue in leucyl-tRNA synthetase affecting amino acid specificity and tRNA aminoacylation (2007), Biochemistry, 46, 4466-4472.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.4

Protein Variants

Protein Variants Comment Organism
K600F the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, the mutant demonstrates a 9fold decrease in its ability to distinguish between leucine and isoleucine effectively, the activity is reduced compared to the wild-type enzyme Homo sapiens
K600L the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, the mutant demonstrates an 11fold increase in its ability to distinguish between leucine and isoleucine effectively, the activity is reduced compared to the wild-type enzyme Homo sapiens
K600R the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, the mutant shows a slight decrease in activity compared to the wild-type enzyme Homo sapiens
L570F the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, it shows a catalytic turnover for isoleucine decreased by a factor of 2, L570F has an 11fold higher Km for leucine compared to the wild-type enzyme, the activity is reduceDdcompared to the wild-type enzyme Escherichia coli
L570K the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, it shows a catalytic turnover for isoleucine decreased by a factor of 2, L570F has an 11fold higher Km for leucine compared to the wild-type enzyme, the activity is reduced compared to the wild-type enzyme Escherichia coli
L570R the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, it shows a catalytic turnover for isoleucine decreased by a factor of 2, L570R has a 4fold stronger binding affinity for leucine compared to the wild-type enzyme, the activity is reduce compared to the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000018
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant L570K Escherichia coli
0.00016
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Escherichia coli
0.0002
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Homo sapiens
0.0004
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Escherichia coli
0.0015
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant K600L Homo sapiens
0.0017
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570F Escherichia coli
0.0017
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant L570F Escherichia coli
0.0018
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutants K600L and K600R Homo sapiens
0.002
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570R Escherichia coli
0.0022
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600F Homo sapiens
0.004
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant K600R Homo sapiens
0.004
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Homo sapiens
0.006
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant K600F Homo sapiens
0.025
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570K Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Escherichia coli
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu Homo sapiens
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu
-
 Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu human mitochondrial LeuRS achieves high aminoacylation fidelity without a functional editing active site, representing a rare example of a class I aminoacyl-tRNA synthetase that does not proofread its products, K600 strongly impacts aminoacylation in two ways: it affects both amino acid discrimination and tRNA binding, overview Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu L570 strongly impacts aminoacylation in two ways: it affects both amino acid discrimination and tRNA binding, overview Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeuCUN
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeuCUN
-
 ?
ATP + L-leucine + tRNALeuCUN
-
 Homo sapiens AMP + diphosphate + L-leucyl-tRNALeuCUN
-
 ?
ATP + L-leucine + tRNALeuUUR
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeuUUR
-
 ?
ATP + L-leucine + tRNALeuUUR
-
 Homo sapiens AMP + diphosphate + L-leucyl-tRNALeuUUR
-
 ?

Synonyms

Synonyms Comment Organism
Leucyl-tRNA synthetase
-
 Escherichia coli
Leucyl-tRNA synthetase
-
 Homo sapiens
LeuRS
-
 Escherichia coli
LeuRS
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000018
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant L570K Escherichia coli
0.00016
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Escherichia coli
0.0017
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant L570F Escherichia coli
0.003
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant K600L Homo sapiens
0.09
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Homo sapiens
0.13
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant K600F Homo sapiens
0.14
-
 tRNALeuUUR human derived substrate, pH 7.0, 37°C, recombinant mutant K600R Homo sapiens
0.35
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600L Homo sapiens
0.39
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Homo sapiens
3
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600F Homo sapiens
3.2
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600R Homo sapiens
9.8
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570K Escherichia coli
14.5
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme Escherichia coli
21
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570R Escherichia coli
24
-
 tRNALeuCUN Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570F Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 assay at Escherichia coli
7 7.5 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Homo sapiens