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Literature summary for 6.1.1.4 extracted from
- High-level expression and single-step purification of leucyl-tRNA synthetase from Aquifex aeolicus (2004), Protein Expr. Purif., 36, 146-149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli BL21-Codon Plus (DE3) cells are co-transformed with plasmid pSBETH6-lrsa and pET-15b-lrsb to produce alphabeta-LeuRS with an N-terminal His6 tag | Aquifex aeolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00032 | - |
tRNALeu(GAG) | 65°C, recombinant His6-tagged enzyme | Aquifex aeolicus | |
| 0.00045 | - |
tRNALeu(GAG) | 65°C, wild-type enzyme | Aquifex aeolicus | |
| 0.0064 | - |
L-leucine | 65°C, recombinant His6-tagged enzyme | Aquifex aeolicus |  |
| 0.0064 | - |
L-leucine | 65°C, wild-type enzyme | Aquifex aeolicus | |
| 0.112 | - |
ATP | 65°C, wild-type enzyme | Aquifex aeolicus | |
| 0.113 | - |
ATP | 65°C, recombinant His6-tagged enzyme | Aquifex aeolicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| single-step purification | Aquifex aeolicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.17 | - |
- |
Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu(GAG) | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu(GAG) | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.48 | - |
ATP | 65°C, wild-type enzyme | Aquifex aeolicus | |
| 1.48 | - |
tRNALeu(GAG) | 65°C, wild-type enzyme | Aquifex aeolicus | |
| 1.57 | - |
L-leucine | 65°C, wild-type enzyme | Aquifex aeolicus | |
| 1.59 | - |
tRNALeu(GAG) | 65°C, recombinant His6-tagged enzyme | Aquifex aeolicus | |
| 1.79 | - |
L-leucine | 65°C, recombinant His6-tagged enzyme | Aquifex aeolicus | |
| 2.2 | - |
ATP | 65°C, recombinant His6-tagged enzyme | Aquifex aeolicus | |
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