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Literature summary for 6.1.1.4 extracted from

  • Zheng, Y.G.; Wei, H.; Ling, C.; Martin, F.; Eriani, G.; Wang, E.D.
    Two distinct domains of the beta subunit of Aquifex aeolicus leucyl-tRNA synthetase are involved in tRNA binding as revealed by a three-hybrid selection (2004), Nucleic Acids Res., 32, 3294-3303.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of alpha subunit and beta subunit in Escherichia coli Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
A156V mutation in beta-subunit, the ratio of turnover-number to Km-value is identical to the wild-type ratio Aquifex aeolicus
G237D mutation in beta-subunit, the ratio of turnover-number to Km-value is 1.7fold higher than the wild-type ratio Aquifex aeolicus
K238A mutation in beta-subunit, the ratio of turnover-number to Km-value is 2.4fold higher than the wild-type ratio Aquifex aeolicus
L283F mutation in beta-subunit, the ratio of turnover-number to Km-value is 2fold higher than the wild-type ratio Aquifex aeolicus
M159A mutation in beta-subunit, the ratio of turnover-number to Km-value is 80% of the wild-type value Aquifex aeolicus
M160N mutation in beta-subunit, the ratio of turnover-number to Km-value is 2.4fold higher than the wild-type ratio Aquifex aeolicus
N152A mutation in beta-subunit, the ratio of turnover-number to Km-value is 16% of the wild-type ratio Aquifex aeolicus
N163A mutation in beta-subunit, the ratio of turnover-number to Km-value is 2.4fold higher than the wild-type ratio Aquifex aeolicus
Q234H mutation in beta-subunit, the ratio of turnover-number to Km-value is identical to the wild-type ratio Aquifex aeolicus
Q260stop mutation in beta-subunit, the ratio of turnover-number to Km-value is 0.02% of the wild-type ratio Aquifex aeolicus
R94A mutation in beta-subunit, the ratio of turnover-number to Km-value is 40% of the wild-type ratio Aquifex aeolicus
V286stop mutation in beta-subunit, the ratio of turnover-number to Km-value is 0.6% of the wild-type ratio Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 tRNALeu 37°C, pH 7.8, mutant enzyme N163A Aquifex aeolicus
0.00011
-
 tRNALeu 37°C, pH 7.8, mutant enzyme K238A Aquifex aeolicus
0.00014
-
 tRNALeu 37°C, pH 7.8, mutant enzyme G237D Aquifex aeolicus
0.00018
-
 tRNALeu 37°C, pH 7.8, mutant enzyme L283F Aquifex aeolicus
0.0002
-
 tRNALeu 37°C, pH 7.8, wilde-type enzyme Aquifex aeolicus
0.00024
-
 tRNALeu 37°C, pH 7.8, mutant enzyme K160N Aquifex aeolicus
0.00025
-
 tRNALeu 37°C, pH 7.8, mutant enzyme Q234H Aquifex aeolicus
0.00029
-
 tRNALeu 37°C, pH 7.8, mutant enzyme N152A Aquifex aeolicus
0.00038
-
 tRNALeu 37°C, pH 7.8, mutant enzyme M159A Aquifex aeolicus
0.0005
-
 tRNALeu 37°C, pH 7.8, mutant enzyme R94A Aquifex aeolicus
0.00051
-
 tRNALeu 37°C, pH 7.8, mutant enzyme A156V Aquifex aeolicus
0.0035
-
 tRNALeu 37°C, pH 7.8, mutant enzyme V286stop Aquifex aeolicus
0.0095
-
 tRNALeu 37°C, pH 7.8, mutant enzyme Q269stop Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu two distinct domains of the beta subunit of Aquifex aeolicus leucyl-tRNA synthetase are involved in tRNA binding as revealed by a three-hybrid selection Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

Subunits Comment Organism
dimer heterodimer Aquifex aeolicus

Synonyms

Synonyms Comment Organism
alphabeta-LeuRS
-
 Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
 tRNALeu 37°C, pH 7.8, mutant enzyme Q269stop Aquifex aeolicus
0.072
-
 tRNALeu 37°C, pH 7.8, mutant enzyme N152A Aquifex aeolicus
0.31
-
 tRNALeu 37°C, pH 7.8, mutant enzyme R94A Aquifex aeolicus
0.32
-
 tRNALeu 37°C, pH 7.8, mutant enzyme V286stop Aquifex aeolicus
0.32
-
 tRNALeu 37°C, pH 7.8, wild-type enzyme Aquifex aeolicus
0.39
-
 tRNALeu 37°C, pH 7.8, mutant enzyme G237D Aquifex aeolicus
0.39
-
 tRNALeu 37°C, pH 7.8, mutant enzyme N163A Aquifex aeolicus
0.4
-
 tRNALeu 37°C, pH 7.8, mutant enzyme Q234H Aquifex aeolicus
0.42
-
 tRNALeu 37°C, pH 7.8, mutant enzyme K238A Aquifex aeolicus
0.47
-
 tRNALeu 37°C, pH 7.8, mutant enzyme M159A Aquifex aeolicus
0.59
-
 tRNALeu 37°C, pH 7.8, mutant enzyme L283F Aquifex aeolicus
0.66
-
 tRNALeu 37°C, pH 7.8, mutant enzyme K160N Aquifex aeolicus
0.84
-
 tRNALeu 37°C, pH 7.8, mutant enzyme A156V Aquifex aeolicus