Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.4 extracted from

  • Ling, C.; Yao, Y.N.; Zheng, Y.G.; Wei, H.; Wang, L.; Wu, X.F.; Wang, E.D.
    The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex (2005), J. Biol. Chem., 280, 34755-34763.
    View publication on PubMed
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of hcLeuRS and DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) in a baculovirus system Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.0019
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, full-length enzyme Homo sapiens
0.0058
-
 L-leucine 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.0075
-
 L-leucine 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
0.064
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.075
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens
0.725
-
 ATP 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.773
-
 ATP 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
1.308
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens
1.349
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol predominantly Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 x * 135000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Homo sapiens human cytosolic leucyl-tRNA synthetase is one component of a macromolecular aminoacyl-tRNA synthetase complex. The C-terminal peptide of hcLeuRS is critical for the interaction with hcArgRS and the interaction in the multi-tRNA synthetase complex AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu human cytosolic leucyl-tRNA synthetase is one component of a macromolecular aminoacyl-tRNA synthetase complex. The C-terminal peptide of hcLeuRS is critical for the interaction with hcArgRS and the interaction in the multi-tRNA synthetase complex Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

Subunits Comment Organism
? x * 135000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
HcleuRS
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, full-length enzyme Homo sapiens
0.059
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.27
-
 ATP 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
0.3
-
 L-leucine 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
0.55
-
 ATP 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.56
-
 L-leucine 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.74
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.79
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens
0.81
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
0.82
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens