Protein Variants | Comment | Organism |
---|---|---|
T252A | the ratio of turnover-number to KM-value for L-leucine in aminoacylation is 5% of the wild-type ratio, the ratio of turnover-number to KM-value for tRNALeu in aminoacylation is 10% of the wild-type ratio. The ratio of turnover-number to Km value for Leu-tRNALeu(UAA) is 25fold higher than the wild-type value, the ratio of turnover-number to Km value for Ile-tRNALeu(UAA) is 1.25fold higher than the wild-type value | Escherichia coli |
T252S | the ratio of turnover-number to KM-value for L-leucine in aminoacylation is 60% of the wild-type ratio, the ratio of turnover-number to KM-value for tRNALeu in aminoacylation is 80% of the wild-type ratio. The ratio of turnover-number to Km value for Leu-tRNALeu(UAA) is 7.5fold higher than the wild-type value, the ratio of turnover-number to Km value for Ile-tRNALeu(UAA) is 1.6fold lower than the wild-type value | Escherichia coli |
T252V | the ratio of turnover-number to KM-value for L-leucine in aminoacylation is identical to wild-type ratio, the ratio of turnover-number to KM-value for tRNALeu in aminoacylation is 90% of the wild-type ratio. The ratio of turnover-number to Km value for Leu-tRNALeu(UAA) is 1.75fold higher than the wild-type value, the ratio of turnover-number to Km value for Ile-tRNALeu(UAA) is 13.3fold higher than the wild-type value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
tRNALeu(UAA) | wild-type enzyme, aminoacylation | Escherichia coli | |
0.0015 | - |
tRNALeu(UAA) | mutant enzyme T252V, aminoacylation | Escherichia coli | |
0.0015 | - |
L-leucine | wild-type enzyme, aminoacylation | Escherichia coli | |
0.0016 | - |
L-leucine | mutant enzyme T252V, aminoacylation | Escherichia coli | |
0.0017 | - |
tRNALeu(UAA) | mutant enzyme T252S, aminoacylation | Escherichia coli | |
0.002 | - |
L-leucine | mutant enzyme T252S, aminoacylation | Escherichia coli | |
0.0028 | - |
tRNALeu(UAA) | mutant enzyme T252A, aminoacylation | Escherichia coli | |
0.0036 | - |
L-leucine | mutant enzyme T252A, aminoacylation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu(UAA) | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu(UAA) | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
L-leucine | mutant enzyme T252A, aminoacylation | Escherichia coli | |
1.6 | - |
tRNALeu(UAA) | mutant enzyme T252A, aminoacylation | Escherichia coli | |
5.1 | - |
L-leucine | mutant enzyme T252S, aminoacylation | Escherichia coli | |
6.1 | - |
L-leucine | wild-type enzyme, aminoacylation | Escherichia coli | |
6.1 | - |
tRNALeu(UAA) | wild-type enzyme, aminoacylation | Escherichia coli | |
6.2 | - |
L-leucine | mutant enzyme T252V, aminoacylation | Escherichia coli | |
6.3 | - |
tRNALeu(UAA) | mutant enzyme T252V, aminoacylation | Escherichia coli | |
6.5 | - |
tRNALeu(UAA) | mutant enzyme T252S, aminoacylation | Escherichia coli |