Protein Variants | Comment | Organism |
---|---|---|
T252D | mutation results in isoleucylation of tRNALeu, editing activity is impaired, ATP hydrolysis in presence of norvaline is 27% of the wild-type value, ATP hydrolysis in presence of leucine is 98% of the wild-type value | Escherichia coli |
T252E | mutation results in isoleucylation of tRNALeu, editing activity is impaired, ATP hydrolysis in presence of norvaline is 18% of the wild-type value, ATP hydrolysis in presence of leucine is 86% of the wild-type value | Escherichia coli |
T252G | the mutant enzyme, like the native enzyme, does not mischarge tRNALeu with isoleucine, ATP hydrolysis in presence of norvaline is 2.1fold higher than wild-type value, ATP hydrolysis in presence of leucine is 60% of the wild-type value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
tRNALeu | 37°C, pH 7.8, mutant enzyme T25D | Escherichia coli | |
0.0026 | - |
tRNALeu | 37°C, pH 7.8, native enzyme | Escherichia coli | |
0.0033 | - |
tRNALeu | 37°C, pH 7.8, mutant enzyme T252E | Escherichia coli | |
0.019 | - |
L-leucine | 37°C, pH 7.8, mutant enzyme T252E | Escherichia coli | |
0.019 | - |
L-leucine | 37°C, pH 7.8, mutant enzyme T25D | Escherichia coli | |
0.02 | - |
L-leucine | 37°C, pH 7.8, native enzyme | Escherichia coli | |
0.22 | - |
ATP | 37°C, pH 7.8, mutant enzyme T252E | Escherichia coli | |
0.23 | - |
ATP | 37°C, pH 7.8, mutant enzyme T25D | Escherichia coli | |
0.24 | - |
ATP | 37°C, pH 7.8, native enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNALeu | activity with mutant enzymes T252E and T252D, no activity with wild-type enzyme and with mutant enzyme T252G | Escherichia coli | AMP + diphosphate + L-isoleucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Leucyl-tRNA synthetase | - |
Escherichia coli |
LeuRS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
L-leucine | 37°C, pH 7.8, mutant enzyme T25D | Escherichia coli | |
4.7 | - |
tRNALeu | 37°C, pH 7.8, mutant enzyme T25D | Escherichia coli | |
4.8 | - |
ATP | 37°C, pH 7.8, mutant enzyme T25D | Escherichia coli | |
4.9 | - |
ATP | 37°C, pH 7.8, mutant enzyme T252E | Escherichia coli | |
4.9 | - |
L-leucine | 37°C, pH 7.8, mutant enzyme T252E | Escherichia coli | |
5 | - |
ATP | 37°C, pH 7.8, native enzyme | Escherichia coli | |
5.1 | - |
tRNALeu | 37°C, pH 7.8, mutant enzyme T252E | Escherichia coli | |
5.1 | - |
L-leucine | 37°C, pH 7.8, native enzyme | Escherichia coli | |
5.1 | - |
tRNALeu | 37°C, pH 7.8, native enzyme | Escherichia coli |