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Literature summary for 6.1.1.4 extracted from

  • Chen, J.; Li, Y.; Wang, E.; Wang, Y.
    High-level expression and single-step purification of leucyl-tRNA synthetase from Escherichia coli (1999), Protein Expr. Purif., 15, 115-120.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene leuS, overexpression in strain JM109(DE3) as His-tagged protein Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
tRNALeu recombinant and native enzyme Escherichia coli
0.015
-
L-leucine recombinant and native enzyme Escherichia coli
0.26
-
ATP native enzyme Escherichia coli
0.28
-
ATP recombinant enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98500
-
x * 98500, recombinant enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Escherichia coli
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain JM109(DE3), 4.6fold to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.54
-
purified recombinant enzyme Escherichia coli
1.69
-
purified native enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
?

Subunits

Subunits Comment Organism
? x * 98500, recombinant enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
Leucine translase
-
Escherichia coli
Leucine--tRNA ligase
-
Escherichia coli
Leucyl-transfer ribonucleate synthetase
-
Escherichia coli
Leucyl-transfer ribonucleic acid synthetase
-
Escherichia coli
Leucyl-transfer RNA synthetase
-
Escherichia coli
Leucyl-tRNA synthetase
-
Escherichia coli
LeuRS
-
Escherichia coli
Synthetase, leucyl-transfer ribonucleate
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
L-leucine recombinant enzyme Escherichia coli
3.4
-
tRNALeu recombinant enzyme Escherichia coli
3.4
-
L-leucine native enzyme Escherichia coli
3.6
-
ATP recombinant enzyme Escherichia coli
3.9
-
tRNALeu native enzyme Escherichia coli
4.2
-
ATP native enzyme Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli