Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.4 extracted from

  • Du, X.; Wang, E.D.
    E292 is important for the aminoacylation activity of Escherichia coli leucyl-tRNA synthetase (2003), J. Protein Chem., 22, 71-76.
    View publication on PubMed
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutants Escherichia coli

Protein Variants

Protein Variants Comment Organism
E292A unaltered specific activity in amino acid activation reaction, 61% reduced aminoacylation activity compared to the wild-type Escherichia coli
E292D unaltered specific activity in amino acid activation reaction, 53% reduced aminoacylation activity compared to the wild-type Escherichia coli
E292F unaltered specific activity in amino acid activation reaction, 60% reduced aminoacylation activity compared to the wild-type Escherichia coli
E292K unaltered specific activity in amino acid activation reaction, 85% reduced aminoacylation activity compared to the wild-type Escherichia coli
E292Q unaltered specific activity in amino acid activation reaction, 54% reduced aminoacylation activity compared to the wild-type Escherichia coli
E292S unaltered specific activity in amino acid activation reaction, 66% reduced aminoacylation activity compared to the wild-type Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ATP at high concentration, the mutants are more sensisitve than the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 tRNALeu mutant E292K, pH 7.8, 37°C Escherichia coli
0.0021
-
 tRNALeu mutant E292A, pH 7.8, 37°C Escherichia coli
0.0024
-
 tRNALeu mutant E292S, pH 7.8, 37°C Escherichia coli
0.0025
-
 tRNALeu wild-type enzyme, pH 7.8, 37°C Escherichia coli
0.0031
-
 tRNALeu mutant E292Q, pH 7.8, 37°C Escherichia coli
0.004
-
 tRNALeu mutants E292D and E292F, pH 7.8, 37°C Escherichia coli
0.012
-
 L-leucine mutant E292K, pH 7.8, 37°C Escherichia coli
0.013
-
 L-leucine mutant E292S, pH 7.8, 37°C Escherichia coli
0.014
-
 L-leucine mutant E292D, E292A, and E292F pH 7.8, 37°C Escherichia coli
0.015
-
 L-leucine wild-type enzyme and mutant E292Q, pH 7.8, 37°C Escherichia coli
0.08
-
 ATP mutant E292F, pH 7.8, 37°C Escherichia coli
0.1
-
 ATP mutants E292Q, E292D, and E292A, pH 7.8, 37°C Escherichia coli
0.11
-
 ATP mutants E292D and E292K, pH 7.8, 37°C Escherichia coli
0.26
-
 ATP wild-type enzyme, pH 7.8, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Escherichia coli
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from overproducing strain Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu residue E292 is important for aminoacylation activity Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 aminoacid activation activities of wild-type and mutant enzymes Escherichia coli
0.05
-
 recombinant mutant E292K, pH 7.8, 37°C Escherichia coli
0.12
-
 recombinant mutant E292S, pH 7.8, 37°C Escherichia coli
0.14
-
 recombinant mutants E292F and E292A, pH 7.8, 37°C Escherichia coli
0.16
-
 recombinant mutant E292Q, pH 7.8, 37°C Escherichia coli
0.17
-
 recombinant mutant E292D, pH 7.8, 37°C Escherichia coli
0.35
-
 recombinant wild-type enzyme, pH 7.8, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Synonyms

Synonyms Comment Organism
Leucine translase
-
 Escherichia coli
Leucine--tRNA ligase
-
 Escherichia coli
Leucyl-transfer ribonucleate synthetase
-
 Escherichia coli
Leucyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
Leucyl-transfer RNA synthetase
-
 Escherichia coli
Leucyl-tRNA synthetase
-
 Escherichia coli
LeuRS
-
 Escherichia coli
Synthetase, leucyl-transfer ribonucleate
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 ATP mutant E292K, pH 7.8, 37°C Escherichia coli
0.8
-
 L-leucine mutant E292K, pH 7.8, 37°C Escherichia coli
0.8
-
 tRNALeu mutant E292K, pH 7.8, 37°C Escherichia coli
1.5
-
 ATP mutant E292S, pH 7.8, 37°C Escherichia coli
1.6
-
 L-leucine mutant E292S, pH 7.8, 37°C Escherichia coli
1.6
-
 tRNALeu mutant E292S, pH 7.8, 37°C Escherichia coli
1.8
-
 tRNALeu mutant E292A, pH 7.8, 37°C Escherichia coli
1.8
-
 ATP mutant E292F, pH 7.8, 37°C Escherichia coli
1.8
-
 L-leucine mutant E292F, pH 7.8, 37°C Escherichia coli
1.9
-
 L-leucine mutant E292A, pH 7.8, 37°C Escherichia coli
1.9
-
 tRNALeu mutant E292Q, pH 7.8, 37°C Escherichia coli
2
-
 tRNALeu mutant E292F, pH 7.8, 37°C Escherichia coli
2
-
 ATP mutants E292Q and E292A, pH 7.8, 37°C Escherichia coli
2.2
-
 ATP mutant E292D, pH 7.8, 37°C Escherichia coli
2.2
-
 tRNALeu mutant E292D, pH 7.8, 37°C Escherichia coli
2.2
-
 L-leucine mutant E292Q, pH 7.8, 37°C Escherichia coli
2.4
-
 L-leucine mutant E292D, pH 7.8, 37°C Escherichia coli
4.9
-
 ATP wild-type enzyme, pH 7.8, 37°C Escherichia coli
5
-
 tRNALeu wild-type enzyme, pH 7.8, 37°C Escherichia coli
5.1
-
 L-leucine wild-type enzyme, pH 7.8, 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP optimal concentration for the wild-type enzyme is 4 mM, for the mutants 2 mM Escherichia coli