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Literature summary for 6.1.1.4 extracted from

  • Soma, A.; Uchiyama, K.; Sakamoto, T.; Maeda, M.; Himeno, H.
    Unique recognition style of tRNA(Leu) by Haloferax volcanii leucyl-tRNA synthetase (1999), J. Mol. Biol., 293, 1029-1038.
    View publication on PubMed
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
in vitro transcription Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information substrate specificity with diverse tRNALeu isoacceptors and mutants Haloferax volcanii
0.00052
-
 tRNALeuA73 wild-type tRNALeu, pH 7.5, 37°C Haloferax volcanii
0.0014
-
 tRNASer mutant pH 7.5, 37°C Haloferax volcanii
0.0047
-
 tRNALeuU73 tRNALeu isoacceptor, pH 7.5, 37°C Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Haloferax volcanii
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Haloferax volcanii

Reaction

Reaction Comment Organism Reaction ID
ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu recognition of the tRNALeu requires the discriminator base A73 and the long variable arm of appropriate stem length, especially the Haloferax volcanii-specific loop sequence A47CG47D and U47H at the base of the helix Haloferax volcanii
a

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Haloferax volcanii AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeuA73 class II tRNALeu, recognition requires the discriminator base A73 and the long variable arm of appropriate stem length, especially the specific loop sequence A47CG47D and U47H at the base of the helix Haloferax volcanii AMP + diphosphate + L-leucyl-tRNALeuA73
-
 ?
ATP + L-leucine + tRNALeuU73 class II tRNALeu isoacceptor, 17fold lower activity compared to tRNALeuA73 Haloferax volcanii AMP + diphosphate + L-leucyl-tRNALeuU73
-
 ?
ATP + L-leucine + tRNASer mutant transplantation of both the discriminator base and the variable arm of tRNALeu are not sufficient to introduce leucylation activity to tRNASer, but additional insertion of additional a nucleotide into the D-loop, which is not involved in the direct interaction with the enzyme, converts tRNASer to an efficient leucine acceptor Haloferax volcanii AMP + diphosphate + L-leucyl-tRNASer mutant
-
 ?
additional information substrate specificty with diverse class II tRNALeu isoacceptors and mutants, overview, no activity with tRNALeuG73 and C73, no activity with tRNALeu, tRNASer and tRNATyr from Escherichia coli and Saccharomyces cerevisiae, differences in the tertiary structure of tRNALeu and tRNASer play a key role for inactivity and therefore elimination of native tRNASer as leucine acceptor Haloferax volcanii ?
-
 ?

Synonyms

Synonyms Comment Organism
Leucine translase
-
 Haloferax volcanii
Leucine--tRNA ligase
-
 Haloferax volcanii
Leucyl-transfer ribonucleate synthetase
-
 Haloferax volcanii
Leucyl-transfer ribonucleic acid synthetase
-
 Haloferax volcanii
Leucyl-transfer RNA synthetase
-
 Haloferax volcanii
Leucyl-tRNA synthetase
-
 Haloferax volcanii
LeuRS
-
 Haloferax volcanii
Synthetase, leucyl-transfer ribonucleate
-
 Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Haloferax volcanii