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Literature summary for 6.1.1.4 extracted from

  • Zhao, M.W.; Hao, R.; Chen, J.F.; Martin, F.; Eriani, G.; Wang, E.D.
    Enzymes assembled from Aquifex aeolicus and Escherichia coli leucyl-tRNA synthetases (2003), Biochemistry, 42, 7694-7700.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the alpha and beta subunits and the C- and N-terminal parts of the Escherichia coli enzyme in Escherichia coli as monomeric and dimeric mutants, also mixed between the species Aquifex aeolicus
overexpression of the N-terminal and the C-terminal parts of the enzyme and of the alpha- and beta-subunits of the Aquifex aeolicus enzyme in an Escherichia coli strain as monomeric and dimeric mutants, also mixed between the species Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information deletion of the last 36 residues of the alpha-subunit is deleterious for tRNA charging, resulting in an inactive mutant, the monomeric mutants containing the alpha-subunit have activities comparable to the wild-type enzyme, while the heterodimeric enzymes show very low activity Aquifex aeolicus
additional information overexpression of the alpha and beta subunits of the Aquifex aeolicus enzyme and the C- and N-terminal parts of the Escherichia coli enzyme in Escherichia coli as monomeric and dimeric mutants, also mixed between the species, the heterodimeric mutants and the monomeric mutants containing the N-terminal-subpart of the enzyme show very low activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, wild-type and mutant enzymes Escherichia coli
additional information
-
additional information kinetics, wild-type and mutant enzymes Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Escherichia coli
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu Aquifex aeolicus
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activiy of several enzyme mutants and mixed mutants, containing subparts of the Escherichia coli enzyme Escherichia coli
additional information
-
activiy of several enzyme mutants and mixed mutants, containing subparts of the Escherichia coli enzyme Aquifex aeolicus
0.047
-
purified recombinant wild-type enzyme, with substrate tRNALeu from Escherichia coli Aquifex aeolicus
0.235
-
purified recombinant wild-type enzyme, with substrate tRNALeu from Aquifex aeolicus Aquifex aeolicus
1.3
-
purified recombinant wild-type enzyme, with substrate tRNALeu from Aquifex aeolicus Escherichia coli
1.5
-
purified recombinant wild-type enzyme, with substrate tRNALeu from Escherichia coli Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu
-
Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu the cross-species-specific recognition occurs at the alpha-subunit, tRNALeu substrates from Escherichia coli and Aquifex aeolicus Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu tRNALeu substrates from Escherichia coli and Aquifex aeolicus Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
?

Synonyms

Synonyms Comment Organism
Leucine translase
-
Escherichia coli
Leucine translase
-
Aquifex aeolicus
Leucine--tRNA ligase
-
Escherichia coli
Leucine--tRNA ligase
-
Aquifex aeolicus
Leucyl-transfer ribonucleate synthetase
-
Escherichia coli
Leucyl-transfer ribonucleate synthetase
-
Aquifex aeolicus
Leucyl-transfer ribonucleic acid synthetase
-
Escherichia coli
Leucyl-transfer ribonucleic acid synthetase
-
Aquifex aeolicus
Leucyl-transfer RNA synthetase
-
Escherichia coli
Leucyl-transfer RNA synthetase
-
Aquifex aeolicus
Leucyl-tRNA synthetase
-
Escherichia coli
Leucyl-tRNA synthetase
-
Aquifex aeolicus
LeuRS
-
Escherichia coli
LeuRS
-
Aquifex aeolicus
Synthetase, leucyl-transfer ribonucleate
-
Escherichia coli
Synthetase, leucyl-transfer ribonucleate
-
Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the mixed enzyme mutants are not thermostable in contrary to the wild-type enzyme Aquifex aeolicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli
ATP
-
Aquifex aeolicus