Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutants in Escherichia coli strain SG13009 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
T252F | impaired proofreading mechanism, increase rate of misaminoacylation with isoleucine and valine | Escherichia coli |
T252L | impaired proofreading mechanism, increase rate of misaminoacylation with isoleucine and valine | Escherichia coli |
T252Y | impaired proofreading mechanism, increase rate of misaminoacylation with isoleucine and valine, effective aminoacylation of tRNALeu with allylglycine, homopropargylglycine, 2-butynylalanine, norvaline, and norisoleucine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-butynylalanine + tRNALeu | Escherichia coli | aminoacylation by mutant T252Y | AMP + diphosphate + 2-butynylalanyl-tRNALeu | - |
? | |
ATP + allylglycine + tRNALeu | Escherichia coli | aminoacylation by mutant T252Y | AMP + diphosphate + allylglycyl-tRNALeu | - |
? | |
ATP + homoallylglycine + tRNALeu | Escherichia coli | aminoacylation by mutant T252Y | AMP + diphosphate + homoallylglycyl-tRNALeu | - |
? | |
ATP + homopropargylglycine + tRNALeu | Escherichia coli | aminoacylation by mutant T252Y | AMP + diphosphate + homopropargylglycyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | Escherichia coli | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-norisoleucine + tRNALeu | Escherichia coli | aminoacylation by mutant T252Y | AMP + diphosphate + L-norisoleucyl-tRNALeu | - |
? | |
ATP + L-norvaline + tRNALeu | Escherichia coli | aminoacylation by mutant T252Y | AMP + diphosphate + L-norvalyl-tRNALeu | - |
? | |
additional information | Escherichia coli | fidelity of translation is dependent on the specificity of the aminoacyl-tRNA synthetases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant HIs-tagged wild-type and mutant enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-butynylalanine + tRNALeu | aminoacylation by mutant T252Y | Escherichia coli | AMP + diphosphate + 2-butynylalanyl-tRNALeu | - |
? | |
ATP + allylglycine + tRNALeu | aminoacylation by mutant T252Y | Escherichia coli | AMP + diphosphate + allylglycyl-tRNALeu | - |
? | |
ATP + homoallylglycine + tRNALeu | aminoacylation by mutant T252Y | Escherichia coli | AMP + diphosphate + homoallylglycyl-tRNALeu | - |
? | |
ATP + homopropargylglycine + tRNALeu | aminoacylation by mutant T252Y | Escherichia coli | AMP + diphosphate + homopropargylglycyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | two-step reaction, the first step is reversible, the second is not, tRNA discrimination by a double-sieve mechansim | Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-norisoleucine + tRNALeu | aminoacylation by mutant T252Y | Escherichia coli | AMP + diphosphate + L-norisoleucyl-tRNALeu | - |
? | |
ATP + L-norvaline + tRNALeu | aminoacylation by mutant T252Y | Escherichia coli | AMP + diphosphate + L-norvalyl-tRNALeu | - |
? | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction, the enzyme has an editing function to correct misaminoacylation of tRNALeu by isoleucine and methionine, T252 is involved | Escherichia coli | ? | - |
? | |
additional information | fidelity of translation is dependent on the specificity of the aminoacyl-tRNA synthetases | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Leucine translase | - |
Escherichia coli |
Leucine--tRNA ligase | - |
Escherichia coli |
Leucyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Leucyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Leucyl-transfer RNA synthetase | - |
Escherichia coli |
Leucyl-tRNA synthetase | - |
Escherichia coli |
LeuRS | - |
Escherichia coli |
Synthetase, leucyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
aminoacylation assay at | Escherichia coli |
7.6 | - |
ATP-diphosphate exchange assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |