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Literature summary for 6.1.1.4 extracted from

  • Tang, Y.; Tirrell, D.A.
    Attenuation of the editing activity of the Escherichia coli leucyl-tRNA synthetase allows incorporation of novel amino acids into proteins in vivo (2002), Biochemistry, 41, 10635-10645.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutants in Escherichia coli strain SG13009 Escherichia coli

Protein Variants

Protein Variants Comment Organism
T252F impaired proofreading mechanism, increase rate of misaminoacylation with isoleucine and valine Escherichia coli
T252L impaired proofreading mechanism, increase rate of misaminoacylation with isoleucine and valine Escherichia coli
T252Y impaired proofreading mechanism, increase rate of misaminoacylation with isoleucine and valine, effective aminoacylation of tRNALeu with allylglycine, homopropargylglycine, 2-butynylalanine, norvaline, and norisoleucine Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-butynylalanine + tRNALeu Escherichia coli aminoacylation by mutant T252Y AMP + diphosphate + 2-butynylalanyl-tRNALeu
-
?
ATP + allylglycine + tRNALeu Escherichia coli aminoacylation by mutant T252Y AMP + diphosphate + allylglycyl-tRNALeu
-
?
ATP + homoallylglycine + tRNALeu Escherichia coli aminoacylation by mutant T252Y AMP + diphosphate + homoallylglycyl-tRNALeu
-
?
ATP + homopropargylglycine + tRNALeu Escherichia coli aminoacylation by mutant T252Y AMP + diphosphate + homopropargylglycyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu Escherichia coli
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-norisoleucine + tRNALeu Escherichia coli aminoacylation by mutant T252Y AMP + diphosphate + L-norisoleucyl-tRNALeu
-
?
ATP + L-norvaline + tRNALeu Escherichia coli aminoacylation by mutant T252Y AMP + diphosphate + L-norvalyl-tRNALeu
-
?
additional information Escherichia coli fidelity of translation is dependent on the specificity of the aminoacyl-tRNA synthetases ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant HIs-tagged wild-type and mutant enzymes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-butynylalanine + tRNALeu aminoacylation by mutant T252Y Escherichia coli AMP + diphosphate + 2-butynylalanyl-tRNALeu
-
?
ATP + allylglycine + tRNALeu aminoacylation by mutant T252Y Escherichia coli AMP + diphosphate + allylglycyl-tRNALeu
-
?
ATP + homoallylglycine + tRNALeu aminoacylation by mutant T252Y Escherichia coli AMP + diphosphate + homoallylglycyl-tRNALeu
-
?
ATP + homopropargylglycine + tRNALeu aminoacylation by mutant T252Y Escherichia coli AMP + diphosphate + homopropargylglycyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu
-
Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-leucine + tRNALeu two-step reaction, the first step is reversible, the second is not, tRNA discrimination by a double-sieve mechansim Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
?
ATP + L-norisoleucine + tRNALeu aminoacylation by mutant T252Y Escherichia coli AMP + diphosphate + L-norisoleucyl-tRNALeu
-
?
ATP + L-norvaline + tRNALeu aminoacylation by mutant T252Y Escherichia coli AMP + diphosphate + L-norvalyl-tRNALeu
-
?
additional information the enzyme also performs the ATP-diphosphate exchange reaction, the enzyme has an editing function to correct misaminoacylation of tRNALeu by isoleucine and methionine, T252 is involved Escherichia coli ?
-
?
additional information fidelity of translation is dependent on the specificity of the aminoacyl-tRNA synthetases Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
Leucine translase
-
Escherichia coli
Leucine--tRNA ligase
-
Escherichia coli
Leucyl-transfer ribonucleate synthetase
-
Escherichia coli
Leucyl-transfer ribonucleic acid synthetase
-
Escherichia coli
Leucyl-transfer RNA synthetase
-
Escherichia coli
Leucyl-tRNA synthetase
-
Escherichia coli
LeuRS
-
Escherichia coli
Synthetase, leucyl-transfer ribonucleate
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
aminoacylation assay at Escherichia coli
7.6
-
ATP-diphosphate exchange assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli