Literature summary for 6.1.1.3 extracted from

Sankaranarayanan, R.; Dock-Bregeon, A.C.; Romby, P.; Caillet, J.; Springer, M.; Rees, B.; Ehresmann, C.; Ehresmann, B.; Moras, D.
The structure of threonyl-tRNA synthetase-tRNA(Thr) complex enlightens its repressor activity and reveals an essential zinc ion in the active site (1999), Cell, 97, 371-381.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with tRNAThr, hanging drop method, pH 6.5, solution containing ammonium acetate, ATP, and PEG 4000 as precipitant, X-ray diffraction structure determination at 2.9 A resolution and analysis	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	essential, binds at the active site	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-threonine + tRNAThr	Escherichia coli	-	AMP + diphosphate + L-threonyl-tRNAThr	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	class II enzyme, recombinantly overexpressed and purified enzyme	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr	mechanism, active site structure, and substrate recognition method	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-threonine + tRNAThr	-	Escherichia coli	AMP + diphosphate + L-threonyl-tRNAThr	-	?

Synonyms

Synonyms	Comment	Organism
Synthetase, threonyl-transfer ribonucleate	-	Escherichia coli
Threonine translase	-	Escherichia coli
Threonine--tRNA ligase	-	Escherichia coli
Threonine-transfer ribonucleate synthetase	-	Escherichia coli
Threonyl-ribonucleic synthetase	-	Escherichia coli
Threonyl-transfer ribonucleate synthetase	-	Escherichia coli
Threonyl-transfer ribonucleic acid synthetase	-	Escherichia coli
Threonyl-transfer RNA synthetase	-	Escherichia coli
Threonyl-tRNA synthetase	-	Escherichia coli
ThrRS	-	Escherichia coli
TRS	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)