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Literature summary for 6.1.1.3 extracted from
- Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12 (1973), Eur. J. Biochem., 35, 331-337.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | borrelidin-resistant mutant | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12, wild-type and borrelidin-resistant mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for more than 18 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine + tRNAThr | - |
Escherichia coli | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
threonine does not influence the rate of heat inactivation. ATP and tRNA labilize | Escherichia coli |
| 60 | - |
12 min, incubation without substrates, 50% inactivation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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