KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000037 | 0.00007 | tRNAThr | of Thermus thermophilus, , depending on temperature | Thermus thermophilus | |
0.00005 | - |
tRNAThr | of E. coli | Escherichia coli | |
0.000085 | - |
tRNAThr | - |
Escherichia coli | |
0.00014 | - |
tRNAThr | of E. coli | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
2 * 77000, SDS-PAGE under denaturing conditions | Thermus thermophilus |
150000 | - |
- |
Escherichia coli |
150000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.353 | - |
- |
Thermus thermophilus |
Storage Stability | Organism |
---|---|
4°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | - |
Thermus thermophilus | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
ATP + L-threonine + tRNAThr | - |
Escherichia coli | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
ATP + L-threonine + tRNAThr | tRNAThr of Thermus thermophilus | Thermus thermophilus | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
ATP + L-threonine + tRNAThr | tRNAThr3 of Escherichia coli | Escherichia coli | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
ATP + L-threonine + tRNAThr | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-threonyl-tRNAThr | - |
? | |
ATP + L-threonine + tRNAThr | tRNAThr of Thermus thermophilus | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | 2 * 77000, SDS-PAGE under denaturing conditions | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
70 | - |
- |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | 0.23 | tRNAThr | - |
Thermus thermophilus | |
0.21 | - |
tRNAThr | tRNAThr of E. coli | Thermus thermophilus | |
0.52 | 0.7 | tRNAThr | tRNAThr of Thermus thermophilus | Escherichia coli | |
0.53 | - |
tRNAThr | tRNAThr of E. coli | Escherichia coli |