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Literature summary for 6.1.1.27 extracted from
- Phosphoserine aminoacylation of tRNA bearing an unnatural base anticodon (2008), Biochem. Biophys. Res. Commun., 372, 480-485.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D418N/D420N/T423V | site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme, it is active with tRNA substrate containing unusual residues 7-(2-thienyl)-imidazo[4,5-b]pyridine and pyrrole-2-carbaldehyde in the anticodon, overview | Archaeoglobus fulgidus |
| E418N/E420N/T423V | efficiently charged phosphoserine to tRNA containing the (pyrrole-2-carbaldehyde)UA anticodon | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic analysis of Sep-tRNA formation, overview | Archaeoglobus fulgidus | |
| 0.0269 | - |
tRNACys | pH 7.6, 50°C, wild-type enzyme | Archaeoglobus fulgidus |  |
| 0.0372 | - |
tRNACys | pH 7.6, 50°C, mutant D418N/D420N/T423V | Archaeoglobus fulgidus | |
| 0.151 | - |
tRNACys | 50°C, pH 7.6, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon, mutant enzyme D418N D420N T423V | Archaeoglobus fulgidus | |
| 0.151 | - |
tRNACys | 50°C, pH 7.6, tRNACys containing the C(pyrrole-2-carbaldehyde)A anticodon, mutant enzyme D418N D420N T423V | Archaeoglobus fulgidus | |
| 0.269 | - |
tRNACys | 50°C, pH 7.6, tRNACys with the GCA anticodon, wild-type enzyme | Archaeoglobus fulgidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Archaeoglobus fulgidus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + O-phospho-L-serine + tRNACys | Archaeoglobus fulgidus | - |
AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
| ATP + O-phospho-L-serine + tRNACys | Archaeoglobus fulgidus | phosphoseryl-tRNA synthetase is a natural non-standard aminoacyl-tRNA synthetase, which charges a non-standard amino acid, phosphoserine, to tRNACys containing a GCA anticodon for tRNA-dependent cysteine biosynthesis in some archaea | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + O-phospho-L-serine + tRNAAmber | mutant D418N/D420N/T423V | Archaeoglobus fulgidus | AMP + diphosphate + O-phospho-L-seryl-tRNAAmber | - |
? | |
| ATP + O-phospho-L-serine + tRNACys | - |
Archaeoglobus fulgidus | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
| ATP + O-phospho-L-serine + tRNACys | phosphoseryl-tRNA synthetase is a natural non-standard aminoacyl-tRNA synthetase, which charges a non-standard amino acid, phosphoserine, to tRNACys containing a GCA anticodon for tRNA-dependent cysteine biosynthesis in some archaea | Archaeoglobus fulgidus | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
| ATP + O-phospho-L-serine + tRNACys | cognate substrate is tRNACys with the GCA anticodon, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon | Archaeoglobus fulgidus | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
| ATP + O-phospho-L-serine + tRNAOpal | mutant D418N/D420N/T423V | Archaeoglobus fulgidus | AMP + diphosphate + O-phospho-L-seryl-tRNAOpal | - |
? | |
| additional information | substrate specificity, site-specific incorporation of phosphoserine into proteins by mutant D418N/D420N/T423V in response to the 7-(2-thienyl)-imidazo[4,5-b]pyridineUA or C7-(2-thienyl)-imidazo[4,5-b]pyridineA codons within mRNA, substrate binding structures and structural models for tRNA anticodon recognition, overview | Archaeoglobus fulgidus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphoseryl-tRNA synthetase | - |
Archaeoglobus fulgidus |
| phosphoseryl-tRNA synthetases | - |
Archaeoglobus fulgidus |
| SepRS | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Archaeoglobus fulgidus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
tRNACys | pH 7.6, 50°C, mutant D418N/D420N/T423V | Archaeoglobus fulgidus | |
| 0.0471 | - |
tRNACys | 50°C, pH 7.6, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon, mutant enzyme D418N D420N T423V | Archaeoglobus fulgidus | |
| 0.048 | - |
tRNACys | 50°C, pH 7.6, tRNACys containing the C(pyrrole-2-carbaldehyde)A anticodon, mutant enzyme D418N D420N T423V | Archaeoglobus fulgidus | |
| 0.115 | - |
tRNACys | 50°C, pH 7.6, tRNACys with the GCA anticodon, wild-type enzyme | Archaeoglobus fulgidus | |
| 0.115 | - |
tRNACys | pH 7.6, 50°C, wild-type enzyme | Archaeoglobus fulgidus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Archaeoglobus fulgidus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Archaeoglobus fulgidus | |
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Release 2023.1 (January 2023)
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