Cloned (Comment) | Organism |
---|---|
recombinant expression of GFP-tagged mutant enzymes | Methanosarcina sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | liposome-based in vitro compartmentalization (IVC) for LysZRS evolution, directed evolution of LysZ-RS, overview | Methanosarcina sp. |
Y384F/Y306A | site-directed mutagenesis, the mutant can also incorporate L-lysine derivatives N-benzyloxycarbonyl-L-lysines, i.e. LysZ | Methanosarcina sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanosarcina species pyrrolysyl-tRNA synthetase (PylRS) attaches Pyl to its cognate amber suppressor tRNA. The introduction of two mutations (Y384F and Y306A) into PylRS generates a mutant, designated LysZ-RS, that is able to attach N-benzyloxycarbonyl-L-lysine (LysZ) to its cognate tRNA | Methanosarcina sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LysZ-RS | - |
Methanosarcina sp. |
PylRS | - |
Methanosarcina sp. |
pyrrolysyl-tRNA synthetase | - |
Methanosarcina sp. |