Literature summary for 6.1.1.26 extracted from

Heinemann, I.U.; ODonoghue, P.; Madinger, C.; Benner, J.; Randau, L.; Noren, C.J.; Soell, D.
The appearance of pyrrolysine in tRNAHis guanylyltransferase by neutral evolution (2009), Proc. Natl. Acad. Sci. USA, 106, 21103-21108.

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Organism

Organism	UniProt	Comment	Textmining
Methanosarcina acetivorans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-pyrrolysine + tRNAPyl	the Methanosarcina acetivorans tRNAhis guanylyltranferase Thg1 gene contains an in-frame TAG codon. Its presence in Methanosarcina mRNA may lead to pyrrolysine incorporation achieved by Pyl-tRNAPyl, the product of pyrrolysyl-tRNA synthetase. Translation of Thg1 mRNA leads to a full-length, Pyl-containing, active enzyme as determined by immunoblotting, mass spectrometry, and biochemical analysis	Methanosarcina acetivorans	AMP + diphosphate + L-pyrrolysyl-tRNAPyl	-	?

General Information

General Information	Comment	Organism
physiological function	pyrrolysine insertion is akin to natural suppression and unlike the active stop codon reassignment that is required for selenocysteine insertion. In Thg1, pyrrolysine is a dispensable residue that appears to confer no selective advantage	Methanosarcina acetivorans

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Release 2023.1 (January 2023)