Application | Comment | Organism |
---|---|---|
drug development | the apicoplast indirect aminoacylation pathway is a potential drug target | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
gene Pf3D7_1357200, recombinant expression of codon-optimized GluRS in Escherichia coli KRX cells | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | - |
Plasmodium falciparum | 20011 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | Plasmodium falciparum | - |
AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | Plasmodium falciparum | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
additional information | Plasmodium falciparum | the glutamyl residue of Glu-tRNAGln is then transamidated by a glutamyl-tRNAGln amidotransferase (Glu-AdT) in the presence of ATP using Gln as an amide donor, producing GlntRNAGln, coupled reaction assay | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IDD3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | - |
Plasmodium falciparum | AMP + diphosphate + L-glutamyl-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | - |
Plasmodium falciparum | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? | |
additional information | the glutamyl residue of Glu-tRNAGln is then transamidated by a glutamyl-tRNAGln amidotransferase (Glu-AdT) in the presence of ATP using Gln as an amide donor, producing GlntRNAGln, coupled reaction assay | Plasmodium falciparum | ? | - |
? | |
additional information | the non-discriminating GluRS (ND-GluRS) can glutamylate both tRNAGlu and tRNAGln | Plasmodium falciparum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ND-GluRS | - |
Plasmodium falciparum |
non-discriminating GluRS | - |
Plasmodium falciparum |
non-discriminating glutamyl-tRNA synthetase | - |
Plasmodium falciparum |
Pf3D7_1357200 | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
metabolism | the malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a non-discriminating glutamyl-tRNA synthetase to synthesize Glu-tRNAGln and a glutaminyl-tRNA amidotransferase to convert Glu-tRNAGln to Gln-tRNAGln | Plasmodium falciparum |
physiological function | Plasmodium apicoplast protein synthesis is essential for parasite survival, aminoacyl-tRNA formation is essential for protein synthesis. The malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a non-discriminating glutamyl-tRNA synthetase to synthesize Glu-tRNAGln and a glutaminyl-tRNA amidotransferase to convert Glu-tRNAGln to Gln-tRNAGln. Formation of apicoplast Gln-tRNAGln proceeds via indirect aminoacylation. The nucleus-encoded non-discriminating GluRS is imported into the apicoplast is responsible for the formation of misacylated Glu-tRNAGln and is essential in the erythrocytic stages | Plasmodium falciparum |