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Literature summary for 6.1.1.23 extracted from

  • Bailly, M.; Blaise, M.; Lorber, B.; Thirup, S.; Kern, D.
    Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation (2009), Acta Crystallogr. Sect. F, 65, 577-581.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli cells Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
a complete dataset of the transamidosome is collected to 3 A resolution Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
transamidosome the partners involved in tRNA-dependent Asn formation assemble into a ternary complex called the transamidosome, consisting of the ND-AspRS, GatCAD amidotransferase, and tRNAAsn Thermus thermophilus
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn, theoretical Thermus thermophilus
380000
-
ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn, determined by gel filtration Thermus thermophilus
400000
-
ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn, determined by static light scattering, SLS Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-aspartate + tRNAAsn Thermus thermophilus Thermus thermophilus deprived of asparagine synthetase snthesizes Asn on tRNAAsn via a tRNA-dependent pathway involving a nondiscriminating aspartyl-tRNA synthetase that charges Asp onto tRNAAsn prior to conversion of the Asp to Asn by GatCAB AMP + diphosphate + L-aspartyl-tRNAAsn
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
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-
-

Purification (Commentary)

Purification (Comment) Organism
the transamidosome complex is isolated by preparative gel filtration on a Superdex G200 column Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsn Thermus thermophilus deprived of asparagine synthetase snthesizes Asn on tRNAAsn via a tRNA-dependent pathway involving a nondiscriminating aspartyl-tRNA synthetase that charges Asp onto tRNAAsn prior to conversion of the Asp to Asn by GatCAB Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsn
-
?

Subunits

Subunits Comment Organism
trimer ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn Thermus thermophilus

Synonyms

Synonyms Comment Organism
Aspartyl-tRNA synthetase
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Thermus thermophilus
ND-AspRS
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Thermus thermophilus
nondiscriminating aspartyl-tRNA synthetase
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Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity measurement of the transamidosome Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
activity measurement of the transamidosome Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermus thermophilus