Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli cells | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
a complete dataset of the transamidosome is collected to 3 A resolution | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
transamidosome | the partners involved in tRNA-dependent Asn formation assemble into a ternary complex called the transamidosome, consisting of the ND-AspRS, GatCAD amidotransferase, and tRNAAsn | Thermus thermophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn, theoretical | Thermus thermophilus |
380000 | - |
ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn, determined by gel filtration | Thermus thermophilus |
400000 | - |
ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn, determined by static light scattering, SLS | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | Thermus thermophilus | Thermus thermophilus deprived of asparagine synthetase snthesizes Asn on tRNAAsn via a tRNA-dependent pathway involving a nondiscriminating aspartyl-tRNA synthetase that charges Asp onto tRNAAsn prior to conversion of the Asp to Asn by GatCAB | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the transamidosome complex is isolated by preparative gel filtration on a Superdex G200 column | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | Thermus thermophilus deprived of asparagine synthetase snthesizes Asn on tRNAAsn via a tRNA-dependent pathway involving a nondiscriminating aspartyl-tRNA synthetase that charges Asp onto tRNAAsn prior to conversion of the Asp to Asn by GatCAB | Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | ternary complex, transamidosome, ND-AspRS-GatCAB-tRNAAsn | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
Aspartyl-tRNA synthetase | - |
Thermus thermophilus |
ND-AspRS | - |
Thermus thermophilus |
nondiscriminating aspartyl-tRNA synthetase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity measurement of the transamidosome | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
activity measurement of the transamidosome | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |