Literature summary for 6.1.1.23 extracted from

Bernard, D.; Akochy, P.M.; Bernier, S.; Fisette, O.; Brousseau, O.C.; Chenevert, R.; Roy, P.H.; Lapointe, J.
Inhibition by L-aspartol adenylate of a nondiscriminating aspartyl-tRNA synthetase reveals differences between the interactions of its active site with tRNAAsp and tRNAAsn (2007), J. Enzyme Inhib. Med. Chem., 22, 77-82.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally His-tagged enzyme in strain ADD1976	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
L-aspartol adenylate	i.e. Asp-ol-AMP, a stable analogue of the natural reaction intermediate L-aspartyl adenylate, biphasic, competitive inhibition, differential inhibition of tRNAAsp and tRNAAsn aspartylation by the enzyme, overview	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate + tRNAAsp	Pseudomonas aeruginosa	-	AMP + diphosphate + aspartyl-tRNAAsp	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from strain ADD1976 by nickel affinity chromatography	Pseudomonas aeruginosa

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + L-aspartate + tRNAAsx = AMP + diphosphate + L-aspartyl-tRNAAsx	via reaction intermediate L-aspartyl adenylate	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate + tRNAAsp	-	Pseudomonas aeruginosa	AMP + diphosphate + aspartyl-tRNAAsp	-	?
ATP + L-aspartate + tRNAAsx	preparation of recombinant tRNA substrates from Pseudomonas aeruginosa and Saccharomyces cerevisiae, overview, activity with two variants C36U and C38U of yeast tRNAAsp	Pseudomonas aeruginosa	AMP + diphosphate + L-aspartyl-tRNAAsx	-	?

Synonyms

Synonyms	Comment	Organism
ND-AspRS	-	Pseudomonas aeruginosa
nondiscriminating aspartyl-tRNA synthetase	-	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics	Pseudomonas aeruginosa
0.041	-	L-aspartol adenylate	tRNAAsp aspartylation	Pseudomonas aeruginosa
0.215	-	L-aspartol adenylate	tRNAAsn aspartylation	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)