Literature summary for 6.1.1.23 extracted from

Becker, H.D.; Roy, H.; Moulinier, L.; Mazauric, M.H.; Keith, G.; Kern, D.
Thermus thermophilus contains an eubacterial and an archaebacterial aspartyl-tRNA synthetase (2000), Biochemistry, 39, 3216-3230.

Search for more literature for 6.1.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00003	-	tRNAAsp	AspRS1	Thermus thermophilus
0.00006	-	tRNAAsn	AspRS2	Thermus thermophilus
0.00007	-	tRNAAsp	AspRS2	Thermus thermophilus
0.005	-	aspartate	AspRS2	Thermus thermophilus
0.03	-	aspartate	AspRS1	Thermus thermophilus
0.033	-	ATP	AspRS2	Thermus thermophilus
0.28	-	ATP	AspRS1	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-aspartate + tRNAAsp	Thermus thermophilus	-	AMP + diphosphate + Asp-tRNAAsp	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
32.1	-	-	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-aspartate + tRNAAsn	only the enzyme AspRS2 aspartylates tRNAAsn	Thermus thermophilus	AMP + diphosphate + Asp-tRNAAsn	-	?
ATP + L-aspartate + tRNAAsp	-	Thermus thermophilus	AMP + diphosphate + Asp-tRNAAsp	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.092	-	tRNAAsn	AspRS2	Thermus thermophilus
0.24	-	tRNAAsp	AspRS2	Thermus thermophilus
7.2	-	tRNAAsp	AspRS1	Thermus thermophilus

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Release 2023.1 (January 2023)