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Literature summary for 6.1.1.22 extracted from
- Purification and some properties of asparagine, lysine, serine, and valine:tRNA ligases from Bacillus stearothermophilus (1978), J. Biol. Chem., 253, 7033-7039.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
2 * 51000, sedimentation equilibrium measurement under denaturing conditions | Geobacillus stearothermophilus |
| 120000 | - |
calculation from gel filtration and sedimentation velocity | Geobacillus stearothermophilus |
| 127000 | - |
sedimentation equilibrium measurement | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.113 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-asparagine + tRNAAsn | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 51000, sedimentation equilibrium measurement under denaturing conditions | Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature-induced conformation change of the enzymes affecting primarily the binding sites for ATP and amino acid | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
3 min, stable, in presence of all substrates except amino acid | Geobacillus stearothermophilus |
| 70 | - |
0.5 min, stable, in presence of all substrates except amino acid | Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 25 C, the aminoacylation rate increases as the pH is raised from 6.0 to 8.0 | Geobacillus stearothermophilus |
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