Literature summary for 6.1.1.22 extracted from

Andrulis, I.L.; Chiang, C.S.; Arfin, S.M.; Miner, T.A.; Hatfield, G.W.
Biochemical characterization of a mutant asparaginyl-tRNA synthetase from Chinese hamster ovary cells (1978), J. Biol. Chem., 253, 58-62.

Search for more literature for 6.1.1.22

Protein Variants

Protein Variants	Comment	Organism
L65A	the mutant strain lys65a produces an enzyme with greater temperature lability in vitro, a higher temperature-independent Km for Asn, and a lower temperature-dependent catalytic capacity than the enzyme from the wild-type strain	Cricetulus griseus

General Stability

General Stability	Organism
ATP and Mg2+ stabilize against thermal inactivation	Cricetulus griseus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00006	-	tRNAAsn	wild-type and mutant enzyme	Cricetulus griseus
0.03	-	Asn	wild-type	Cricetulus griseus
0.1	-	Asn	mutant lys65a	Cricetulus griseus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
152000	-	wild-type and mutant enzyme, sucrose density gradient centrifugation	Cricetulus griseus
155000	-	gel filtration	Cricetulus griseus

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	-	wild-type and temperature-sensitive mutant strain lys65a	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
ovary	-	Cricetulus griseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-asparagine + tRNAAsn	-	Cricetulus griseus	AMP + diphosphate + L-asparaginyl-tRNAAsn	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	wild-type	Cricetulus griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	ATP and Mg2+ stabilize against thermal inactivation	Cricetulus griseus
37	-	wild-type enzyme is stable, mutant enzyme is irreversibly inactivated, half-life: 4.8 min	Cricetulus griseus

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Release 2023.1 (January 2023)