Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
enzyme complexed with tRNAHis, L-histidine, and AMPPNP, hanging drop vapor diffusion method, using 5% (w/v) PEG 4000 and 0.1 M NaOAc (pH4.5) | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
325-328Del | the mutant shows about 20% of wild type activity | Thermus thermophilus |
328insTTET | the mutant shows about 80% of wild type activity | Thermus thermophilus |
E114A | inactive | Thermus thermophilus |
E388A | the mutant shows about 15% of wild type activity | Thermus thermophilus |
E64A | inactive | Thermus thermophilus |
G285A | inactive | Thermus thermophilus |
G286A | the mutant shows about 2% of wild type activity | Thermus thermophilus |
G72V | the mutant shows about 50% of wild type activity | Thermus thermophilus |
G72V/G73V | the mutant shows about 15% of wild type activity | Thermus thermophilus |
K209A | the mutant shows about 80% of wild type activity | Thermus thermophilus |
K397Q | the mutant shows about 10% of wild type activity | Thermus thermophilus |
Q404A | the mutant shows about 90% of wild type activity | Thermus thermophilus |
R115A | the mutant shows about 20% of wild type activity | Thermus thermophilus |
R120A | the mutant shows about 8% of wild type activity | Thermus thermophilus |
R122A | the mutant shows about 15% of wild type activity | Thermus thermophilus |
R122K | the mutant shows about 55% of wild type activity | Thermus thermophilus |
R197A | the mutant shows about 50% of wild type activity | Thermus thermophilus |
R7A | the mutant shows about 20% of wild type activity | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-histidine + tRNAHis | Thermus thermophilus | - |
AMP + diphosphate + L-histidyl-tRNAHis | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P62374 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-histidine + tRNAHis | - |
Thermus thermophilus | AMP + diphosphate + L-histidyl-tRNAHis | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HisRS | - |
Thermus thermophilus |
Histidyl-tRNA synthetase | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |