Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.21 extracted from

  • Yuan, J.; Gogakos, T.; Babina, A.M.; Soell, D.; Randau, L.
    Change of tRNA identity leads to a divergent orthogonal histidyl-tRNA synthetase/tRNAHis pair (2011), Nucleic Acids Res., 39, 2286-2293.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.21

Cloned(Commentary)

Cloned (Comment) Organism
recombinant HisRS allows complete histidylation of a Caulobacter crescentus tRNAHis transcript lacking G-1, addition of G-1 does not improve the aminoacylation Caulobacter vibrioides

Inhibitors

Inhibitors Comment Organism Structure
KCl
-
 Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics with diverse tRNAHIs variants, overview Caulobacter vibrioides
0.00325
-
 tRNAHis wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C Caulobacter vibrioides

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Caulobacter vibrioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52300
-
 x * 52300 Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-histidine + tRNAHis Caulobacter vibrioides
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
additional information Caulobacter vibrioides Caulobacter crescentus HisRS shows very low activity with Escherichia coli tRNAHis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + tRNAHis
-
 Caulobacter vibrioides AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
additional information Caulobacter crescentus HisRS shows very low activity with Escherichia coli tRNAHis Caulobacter vibrioides ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52300 Caulobacter vibrioides

Synonyms

Synonyms Comment Organism
HisRS
-
 Caulobacter vibrioides
Histidyl-tRNA synthetase
-
 Caulobacter vibrioides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Caulobacter vibrioides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 tRNAHis wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C Caulobacter vibrioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Caulobacter vibrioides

General Information

General Information Comment Organism
evolution a conserved tRNA recognition pattern change during evolution leading to a divergent orthogonal HisRS/tRNAHis pair Caulobacter vibrioides
malfunction mutations in the tRNAHis anticodon cause a drastic loss of in vitro histidylation, and mutations of bases A73 and U72 also reduce charging Caulobacter vibrioides
physiological function mature tRNAHis has at its 5'-terminus an extra guanylate, designated as G-1. This is the major recognition element for HisRS to permit acylation of tRNAHis with histidine. However, recombinant HisRS allows complete histidylation of a Caulobacter crescentus tRNAHis transcript lacking G-1, and addition of G-1 does not improve the aminoacylation, but increases Km. The major recognition elements in Caulobacter crescentus tRNAHis are the anticodon, the discriminator base and U72, which are recognized by the divergent, based on sequence similarity, Caulobacter crescentus HisRS, a divergent orthogonal HisRS/tRNAHis pair Caulobacter vibrioides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1231
-
 tRNAHis wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C Caulobacter vibrioides