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Literature summary for 6.1.1.21 extracted from

  • Rosen, A.E.; Brooks, B.S.; Guth, E.; Francklyn, C.S.; Musier-Forsyth, K.
    Evolutionary conservation of a functionally important backbone phosphate group critical for aminoacylation of histidine tRNAs (2006), RNA, 12, 1315-1322.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.21

Protein Variants

Protein Variants Comment Organism
additional information mutational study of the 1:73 base pair recognition by yeats HisRS using two chemically synthesized 24-nucleotide RNA microhelices, overview Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00014
-
 tRNAHis pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
0.0038
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Escherichia coli
0.009
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
0.03
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Escherichia coli
0.034
-
 tRNAHis pH 8.0, 37°C, aminoacylation Escherichia coli
0.124
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
0.5
-
 ATP pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
0.89
-
 ATP pH 8.0, 37°C, diphosphate exchange Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-histidine + tRNAHis Escherichia coli
-
 AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
ATP + L-histidine + tRNAHis Saccharomyces cerevisiae
-
 AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + RNA microhelix
-
 Escherichia coli ?
-
 ?
ATP + L-histidine + RNA microhelix
-
 Saccharomyces cerevisiae ?
-
 ?
ATP + L-histidine + tRNAHis
-
 Escherichia coli AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
ATP + L-histidine + tRNAHis
-
 Saccharomyces cerevisiae AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
ATP + L-histidine + tRNAHis all histidine tRNA molecules have an extra nucleotide, G-1, at the 59 end of the acceptor stem, G-1:C73 base pair binding analysis of the yeast enzyme Saccharomyces cerevisiae AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
ATP + L-histidine + tRNAHis all histidine tRNA molecules have an extra nucleotide, G-1, at the 59 end of the acceptor stem, importance of the G-1:C73 base pair to tRNAHisc, identity, specifically the 59-monophosphate of G-1 and the major groove amine of C73 are recognized by the enzyme of Escherichia coli stabilizing the reaction transition state Escherichia coli AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
additional information binding of a chemically synthesized 24-nucleotide RNA microhelix, which recapitulates the acceptor stem of Escherichia coli tRNAHis, functional group analysis, overview Escherichia coli ?
-
 ?
additional information binding of a chemically synthesized 24-nucleotide RNA microhelix, which recapitulates the acceptor stem of Saccharomyces cervisiae tRNAHis, functional group analysis, overview Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
HisRS
-
 Escherichia coli
HisRS
-
 Saccharomyces cerevisiae
histidyl-transfer RNA synthetase
-
 Escherichia coli
histidyl-transfer RNA synthetase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 aminoacylation assay at Escherichia coli
30
-
 aminoacylation assay at Saccharomyces cerevisiae
37
-
 diphosphate exchange assay at Escherichia coli
37
-
 diphosphate exchange assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0013
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
0.024
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Escherichia coli
0.42
-
 tRNAHis pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
1.71
-
 tRNAHis pH 8.0, 37°C, aminoacylation Escherichia coli
40
-
 ATP pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
40
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
130
-
 ATP pH 8.0, 37°C, diphosphate exchange Escherichia coli
130
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 aminoacylation assay at Escherichia coli
7.5
-
 aminoacylation assay at Saccharomyces cerevisiae
8
-
 diphosphate exchange assay at Escherichia coli
8
-
 diphosphate exchange assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Saccharomyces cerevisiae