Protein Variants | Comment | Organism |
---|---|---|
E83A | 15fold increase in Km-value, decrease in kcat-value | Escherichia coli |
E83Q | 2fold decrease in Km-value, decrease in kcat-value | Escherichia coli |
Q127A | decrease in kcat-value | Escherichia coli |
R259H | 10fold increase in Km-value, decrease in kcat-value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
tRNAHis | mutant E83Q, pH 7.5, 37°C | Escherichia coli | |
0.00034 | - |
tRNAHis | wild-type, pH 7.5, 37°C | Escherichia coli | |
0.00048 | - |
tRNAHis | mutant Q127A, pH 7.5, 37°C | Escherichia coli | |
0.0033 | - |
tRNAHis | mutant R159H, pH 7.5, 37°C | Escherichia coli | |
0.00515 | - |
tRNAHis | mutant E83A, pH 7.5, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60906 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-histidine + tRNAHis = AMP + diphosphate + L-histidyl-tRNAHis | substrate-assisted concerted mechanism, rate of aminoacyl transfer is independent of the interaction between the carboxamide group of Q127 and the alpha-carboxylate carbon | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-histidine + tRNAHis | - |
Escherichia coli | AMP + diphosphate + L-histidyl-tRNAHis | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
tRNAHis | mutant E83A, pH 7.5, 37°C | Escherichia coli | |
0.0006 | - |
tRNAHis | mutant R159H, pH 7.5, 37°C | Escherichia coli | |
0.01 | - |
tRNAHis | mutant E83Q, pH 7.5, 37°C | Escherichia coli | |
0.048 | - |
tRNAHis | mutant Q127A, pH 7.5, 37°C | Escherichia coli | |
2.02 | - |
tRNAHis | wild-type, pH 7.5, 37°C | Escherichia coli |