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Literature summary for 6.1.1.20 extracted from

  • Kartvelishvili, E.; Peretz, M.; Tworowski, D.; Moor, N.; Safro, M.
    Chimeric human mitochondrial PheRS exhibits editing activity to discriminate nonprotein amino acids (2016), Protein Sci., 25, 618-626 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00057
-
L-phenylalanine mitochondrial chimeric enzyme with implanted editing module from Escherichia coli phenylalanine-tRNA synthetase, at pH 8.5 and 37°C Homo sapiens
0.00084
-
L-phenylalanine wild type mitochondrial enzyme, at pH 8.5 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe Homo sapiens
-
AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O95363
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-phenylalanine + tRNAPhe
-
Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
?

Subunits

Subunits Comment Organism
monomer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
Phenylalanine-tRNA synthetase
-
Homo sapiens
PheRS
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
L-phenylalanine mitochondrial chimeric enzyme with implanted editing module from Escherichia coli phenylalanine-tRNA synthetase, at pH 8.5 and 37°C Homo sapiens
0.06
-
L-phenylalanine wild type mitochondrial enzyme, at pH 8.5 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
physiological function cytosolic phenylalanyl-tRNA synthetase exerts control over the translation accuracy, hydrolyzing misacylated products, while the monomeric mitochondrial enzyme lacks the editing activity Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.8
-
L-phenylalanine mitochondrial chimeric enzyme with implanted editing module from Escherichia coli phenylalanine-tRNA synthetase, at pH 8.5 and 37°C Homo sapiens
71.4
-
L-phenylalanine wild type mitochondrial enzyme, at pH 8.5 and 37°C Homo sapiens