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Literature summary for 6.1.1.20 extracted from

  • Gan, R.; Perez, J.G.; Carlson, E.D.; Ntai, I.; Isaacs, F.J.; Kelleher, N.L.; Jewett, M.C.
    Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins (2017), Biotechnol. Bioeng., 114, 1074-1086 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
D286R the mutant shows improvement of activity over the parental enzyme Methanocaldococcus jannaschii
H283T/P284S/M285D/D286V the mutant shows approximate 8fold improvement of activity over the parental enzyme Methanocaldococcus jannaschii
P258A/F261P/H283L/M285F/D286Y the mutant shows improvement of activity over the parental enzyme Methanocaldococcus jannaschii
P284V/D286R the mutant shows improvement of activity over the parental enzyme Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-azido-L-phenylalanine + tRNAPhe Methanocaldococcus jannaschii
-
 AMP + diphosphate + 4-azido-L-phenylalanyl-tRNAPhe
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-azido-L-phenylalanine + tRNAPhe
-
 Methanocaldococcus jannaschii AMP + diphosphate + 4-azido-L-phenylalanyl-tRNAPhe
-
 ?

Synonyms

Synonyms Comment Organism
p-azido-phenylalanyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
pAzFRS
-
 Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanocaldococcus jannaschii