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Literature summary for 6.1.1.20 extracted from
- Tailoring the substrate specificity of yeast phenylalanyl-tRNA synthetase toward a phenylalanine analog using multiple-site-specific incorporation (2015), ACS Synth. Biol., 4, 634-643 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N412G/T415G/S418C/S437F | the mutant prefers 2-L-naphthylalanine as substrate | Saccharomyces cerevisiae |
| T415G | the variant shows 10-fold higher activation activity toward Trp than 2-L-naphthylalanine | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
L-tryptophan | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae |  |
| 2 | - |
2-L-naphthylalanine | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 11 | - |
L-phenylalanine | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-L-naphthylalanine + tRNAPhe | - |
Saccharomyces cerevisiae | AMP + diphosphate + 2-L-naphthylalanyl-tRNAPhe | - |
? | |
| ATP + L-phenylalanine + tRNAPhe | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
| ATP + L-tryptophan + tRNAPhe | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-tryptophanyl-tRNAPhe | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Phenylalanine-tRNA synthetase | - |
Saccharomyces cerevisiae |
| PheRS | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
L-tryptophan | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.0095 | - |
L-phenylalanine | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.03 | - |
2-L-naphthylalanine | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 863 | - |
L-phenylalanine | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 2500 | - |
L-tryptophan | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 15500 | - |
2-L-naphthylalanine | mutant enzyme N412G/T415G/S418C/S437F, at pH 7.6, temperature not specified in the publication | Saccharomyces cerevisiae | |
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